Microsatellite DNA variation in the Siberian sturgeon, Acipenser baeri (Actinopterygii, Acipenseriformes, Acipenseridae), cultured in a Polish fish farm

• Autorzy: Fopp-Bayat D.
• Języki publikacji: EN

Abstrakty

EN

Background. The Siberian sturgeon, Acipenser baeri, is one of the most important sturgeon species cultured in Poland. The effective management of aquaculture production of this species requires contemporary knowledge of broodstock structure, mating patterns, and genetic diversity of broodstock. The aim of the present study was the application of microsatellite DNA analysis for estimation of gene diversity in the Siberian sturgeon farmed at a Polish fish farm. Materials and Methods. Fin clips were randomly sampled from 94 specimens of Siberian sturgeon broodstock reared at the Wąsosze Fish Farm near Konin, Poland. The analysed broodstock has been kept there since 1996, with new specimens being introduced annually. The fish were studied in 2007–2008. Genomic DNA for amplification of microsatellite loci was extracted using Chelex 100. Six microsatellite loci (Afu-19, Afu-39, Afu-68, AfuB-68, Spl-163, and Spl-168) were amplified for examination of the genetic variability of the studied fish. Results. Within 94 individuals of the Siberian sturgeon, a total of 74 alleles were detected in six polymorphic microsatellite loci. The number of alleles per locus ranged from 8 to 18, with an average allele number being 12. The genetic diversity of six microsatellite loci varied from 0.686 to 0.811. Conclusion. This technology has great potential for use in aquaculture of sturgeon fish, especially when levels of genetic variation could be monitored and inbreeding controlled in commercial breeding programs.

Słowa kluczowe

EN

microsatellite DNA; genetic variation; Siberian sturgeon; Acipenser baeri; Actinopterygii; Acipenseriformes; Acipenseridae; Poland; fish farm; animal breeding; aquaculture; molecular marker

• Wydawca: -
• Czasopismo: Acta Ichthyologica et Piscatoria
• Rocznik: 2010
• Tom: 40
• Numer: 1
• Opis fizyczny: p.21-25,fig.,ref.

Twórcy

autor

Fopp-Bayat D.

• Division of Ichthyology, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

Bibliografia

• Anonymous 1994. Red list of threatened animals. Prepared by the IUCN Species Survival Commission. IUCN, Gland, Switzerland.
• Bártfai R., Egedi S., Yue G.H., Kovács B., Urbányi B., Tamás G., Horváth L., Orbán L. 2003. Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers. Aquaculture 219 (1–4): 157–167. DOI: 10.1016/S0044-8486(02)00571-9.
• Birstein V.J., Waldman J.R., Bemis W.E. (eds.) 1997. Sturgeon biodiversity and conservation. Kluwer Academic & Plenum Publishers, New York.
• Blouin M.S., Parsons M., Lacaille V., Lotz S. 1996. Use of microsatellite loci to classify individuals by relatedness. Molecular Ecology 5 (3): 393–401. DOI: 10.1046/j.1365-294X.1996.00094.x.
• Chebanov M., Billard R. 2001. The culture of sturgeons in Russia: production of juveniles for stocking and meat for human consumption. Aquatic Living Resources 14 (6): 375–381. DOI: 10.1016/S0990-7440(01)01122-6.
• Chistiakov D.A., Hellemans B., Haley C.S., Law A.S., Tsigenopoulos C.S., Kotulas G., Bertotto D., Libertini A., Volckaert F.A.M. 2005. A microsatellite linkage map of the European sea bass Dicentrarchus labrax L. Genetics 170: 1821–1826. DOI: 10.1534/genetics.104.039719.
• Clifford S.L., McGinnity P., Ferguson A. 1998. Genetic changes in an Atlantic salmon population resulting from escaped juvenile farm salmon. Journal of Fish Biology 52 (1): 118–127. DOI: 10.1111/j.1095-8649.1998.tb01557.x.
• Desvignes J.F., Laroche J., Durand J.D., Bouvet Y. 2001. Genetic variability in reared stocks of common carp (Cyprinus carpio L.) based on allozymes and microsatellites. Aquaculture 194 (3–4): 291–301. DOI: 10.1016/S0044-8486(00)00534-2.
• Fopp-Bayat D. 2004. Genetic identification of sturgeons, and sturgeon-derived food products: meat and caviar. Archives of Polish Fisheries 12 (Suppl. 2): 231–241.
• Fopp-Bayat D. 2007. Verification of meiotic gynogenesis in Siberian sturgeon (Acipenser baeri Brandt) using microsatellite DNA and cytogenetical markers. Journal of Fish Biology 77 (Supplement C): 478–485. DOI: 10.1111/j.1095-8649.2007.01704.x.
• King T.L., Lubinski B.A., Spidle A.P. 2001. Microsatellite DNA variation in Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and cross-species amplification in the Acipenseridae. Conservation Genetics 2 (2): 103–119. DOI: 10.1023/A:1011895429669.
• May B., Krueger C.C., Kincaid H.L. 1997. Genetic variation at microsatellite loci in sturgeon: primer sequence homology in Acipenser and Scaphirhynchus. Canadian Journal of Fisheries and Aquatic Sciences 54 (7): 1542–1547. DOI: 10.1139/cjfas-54-7-1542.
• McQuown E.C., Sloss B.L., Sheehan R.J., Rodzen J., Tranah G.J., May B. 2000. Microsatellite analysis of genetic variation in sturgeon: New primer sequences for Scaphirhynchus and Acipenser. Transactions of the American Fisheries Society 129:1380–1388. DOI: 10.1577/1548-8659(2000)129<1380:MAOGVI>2.0.CO;2.
• Norris A.T., Bradley D.G., Cunningham E.P. 1999. Microsatellite genetic variation between and within farmed and wild Atlantic salmon (Salmo salar) populations. Aquaculture 180 (3–4): 247–264. DOI: 10.1016/S0044-8486(99)00212-4.
• Park S.D.E. 2001. Trypanotolerance in West African cattle and the population genetic effects of selection. PhD Thesis, University of Dublin.
• Perales-Flores L.E., Sifuentes-Rincón A.M., de León F.J.G. 2007. Microsatellite variability analysis in farmed catfish (Ictalurus punctatus) from Tamaulipas, Mexico. Genetics and Molecular Biology 30 (3): 570–574. DOI: 10.1590/S1415-47572007000400011.
• Pyatskowit J.D., Krueger C.C., Kincaid H.L., May B. 2001. Inheritance of microsatellite loci in the polyploid lake sturgeon (Acipenser fulvescens). Genome 44 (2): 185–191. DOI: 10.1139/gen-44-2-185.
• Su G.-S., Liljedahl L.-E., Gall G.A.E. 1996. Effects of inbreeding on growth and reproductive traits in rainbow trout (Oncorhynchus mykiss). Aquaculture 142 (3–4): 139–148. DOI: 10.1016/0044-8486(96)01255-0.
• Tegelström H. 1986. Mitochondrial DNA in natural populations: an improved routine for the screening of genetic variation based on sensitive silver staining. Electrophoresis 7 (5): 226229. DOI: 10.1002/elps.1150070508.
• Walsh P.S., Metzger D.A., Higuchi R. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506–513.
• Zhu B., Zhou F., Cao H., Shao Z., Zhao N., May B., Chang J. 2002. Analysis of genetic variation in the Chinese sturgeon, Acipenser sinensis: estimating the contribution of artificially produced larvae in a wild population. Journal of Applied Ichthyology 18 (4–6): 301–306. DOI: 10.1046/j.1439-0426.2002.00379.x.