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2002 | 07 | 4 |

Tytuł artykułu

The role of focal adhesion kinase in the emigration of cells from confluent cultures

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
We studied the effect of the modification of focal adhesion kinase (FAK) on the growth, migration and adhesion of C3H 10T1/2 cells. Cells transfected with plasmid coding for antisense FAK displayed a low level of FAK protein. Interestingly, the transfected cells achieved a higher saturation density at confluence, and displayed reduced adhesion and enhanced emigration from a confluent layer of cells when stimulated with fibronectin. In conclusion, it can be postulated that FAK plays an important role in the mechanism of contact inhibition.

Wydawca

-

Rocznik

Tom

07

Numer

4

Opis fizyczny

p.1047-1057,fig.,ref.

Twórcy

autor
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
autor
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
autor
  • Center for Biotechnology, Temple University, 1900N 12th Street, Philadelphia, USA
autor
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland

Bibliografia

  • 1. Abercrombie, M. Contact inhibition and malignancy. Nature 281 (1979) 259-262.
  • 2. Schwartz, M. A. Integrins, oncogenes, and anchorage independence. J. Cell Biol. 139 (1997) 575-578.
  • 3. Ilić, D., Damsky, C. H. and Yamamoto, T. Focal adhesion kinase: at the crossroads of signal transduction. J. Cell Sci. 110 (1997) 401-407.
  • 4. Assoian, R. K. Anchorage- dependent cell cycle progression. Mini-review J. Cell Biol. 136 (1997) 1-4.
  • 5. Reznikoff, C. A., Brankow, D. W., and Heidelberger, C. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to post-confluence inhibition of division. Cancer Res. 33 (1973) 3231-3238.
  • 6. Takeuchi, Y., Suzawa, M., Kikuchi, T., Nishida, E., Fujita, T. and Matsumoto, T. Differentiation and transforming growth factor-β receptor down-regulation by collagen-α2β1 integrin interaction is mediated by focal adhesion kinase and its downstream signals in murine osteoblastic cells. J. Biol. Chem. 272 (1997) 29309-29316.
  • 7. Willumsen, B. M., Papageorge, A. G., Kung, F. J., Bekesi, E., Robins, T., Johnsen, M., Vass, W. and Lowly, D. R. Mutational analysis of a ras catalytic domain. Mol. Cell Biol. 9 (1986) 2646-2654.
  • 8. Janik, P., Szaniawska, B., Kowalczyk, D. and Miłoszewska, J. The effect of phorbol ester treatment on migration of C3H 10T1/2 and BT5C glioma cells: possible application to carcinogenesis. J. Cancer Res. Clin. Oncol. 120 (1994) 156-158.
  • 9. Albini, A., Iwamoto, Y., Kleinman, H. K., Martin, G. R., Aaronson, S. S., Kozlowski, J. M. and McEwan, R. N. A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res. 47 (1987) 3239-3245.
  • 10. Repesh, L. A. A new in vitro assay for ąuantitating tumor cell invasion. Invasion Metastasis 9 (1989) 192-208.
  • 11. Batt, D. B. and Roberts, T. M. Cell density modulates protein-tyrosine phosphorylation. J. Biol. Chem. 273 (1998) 3408-14.
  • 12. Miłoszewska, J., Trawicki, W., Janik, P., Moraczewski, J., Przybyszewska, M. and Szaniawska, B. Protein kinase C translocation in relation to proliferative status of C3H 10T1/2 cells. FEBS Lett. 206 (1986) 283-286.
  • 13. Miłoszewska, J., Trembacz, H. and Janik, P. Signal transduction in confluent C3H10T1/2 cells. The role of focal adhesion kinase. Acta Biochim. Pol. 48 (2001) 175-181.
  • 14. Viñals, F. and Pouysségeur, J. Confluence of vascular endothelial cells induces cell cycle exit by inhibiting p42/p44 mitogen-activated protein kinase activity. Mol. Cell. Biol. 9 (1999) 2763-2772.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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