Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis

• Autorzy: Lirong W. , Pengshan Z. , Xin Z. , Xiaopeng W. , Xiaofei M. , Yi L.
• Języki publikacji: EN

Abstrakty

EN

Braya humilis (Brassicaceae) is a widely distributed plant in arid and semi-arid regions of northern Asia. This plant is well adapted to extremely arid conditions and is a promising candidate species to discover novel drought tolerance strategies. However, not much information about the mechanism(s) mediating drought resistance in this species is currently available. Therefore, the present study aimed to characterize the physiological traits and expression patterns of a polyethylene glycol (PEG)-responsive gene in B. humilis responding to different levels of osmotic stress induced by PEG-6000. Several important physiological parameters were examined, including the levels of relative water content, soluble protein, malondialdehyde, and antioxidant enzyme activity. A tolerance threshold between 20 and 30% PEG-6000 was identified for B. humilis. The water status and oxidative damage below this threshold were maintained at a relatively constant level during the 12 h of treatment. However, once the threshold was exceeded, the water status and oxidative damage were obviously affected after treatment for 4 h. The soluble protein results suggest that B. humilis maintains a vigorous resistance to osmotic stress and that it may play a greater role in osmotic regulation at late stages of stress. Moreover, superoxide dismutase and catalase may be important at preventing oxidative damage in plants at early stages of stress, while peroxidase may be more involved in some biological processes that resist osmotic stress at the late stage, especially in severely damaged plants. Furthermore, a PEG-responsive gene, BhCIPK12, was identified by differential display reverse transcription-polymerase chain reaction (PCR), cloned, and characterized by quantitative real-time PCR. We hypothesized that this gene may play an important role in mediating osmotic stress or drought resistance in plants. Altogether, these results provide valuable insights into the mechanism(s) mediating drought tolerance in B. humilis.

Słowa kluczowe

EN

physiological adaptation; osmotic stress; polyethylene glycol; gene; Braya humilis; drought; expression pattern

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2016
• Tom: 85
• Numer: 1
• Opis fizyczny: Article 3487 [14p.], fig.,ref.

Twórcy

autor

Lirong W.

• College of Forestry Science, Gansu Agricultural University, Lanzhou 730070, China

autor

Pengshan Z.

• Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China

autor

Xin Z.

• Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China

autor

Xiaopeng W.

• Department of Geography, Teachers’ College, Dingxi 743000, China

autor

Xiaofei M.

• Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China

autor

Yi L.

• College of Forestry Science, Gansu Agricultural University, Lanzhou 730070, China

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Identyfikatory

DOI

10.5586/asbp.3487