The role Ttyh1 protein in regulation of dendritic tree and spine formation

• Autorzy: Gorniak-Walas M. , Kaliszewska A. , Lukasiuk K.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Tweety homolog1 (Ttyh1) is a presumed volume-regulated Cl- channel, regulated by cell swelling. Ttyh1 overexpression in dissociated cultures of hippocampal neurons leads to increase in neuritognesis implicating its role in regulation of neuronal morphology. However, the role of Ttyh1 protein in regulation of dendritic tree and spine formation is not well understood. The aim of the study was to examine the influence of Ttyh1 protein in regulation of dendritic tree complexity and formation of dendritic spines in organotypic hippocampal cultures with preserved three dimensional structure. METHODS: Organotypic hippocampal slices were performed from 6 days old rats and transfected with plasmids coding RFP under β-actin promoter and Ttyh1-GFP under synapsin promoter or respective controls, using the Gene Gun. Pyramidal neurons in two areas of the hippocampus: CA1 and CA3 were analyzed using Neuromantic and Sholl software to quantify dendritic tree complexity and dendritic length. SpineMagick software was used to examine dendritic spine morphology and spine density. Proximal and distal parts of apical secondary branches in the stratum radiatum and basal dendrites were analyzed. Dendritic parameters from different groups were compared using a Student’s t test. RESULTS: Sholl analyses did not reveal significant differences in arborization of CA1 and CA3 neurons overexpressing Ttyh1 in comparison to control neurons. Dendritic spine analysis revealed considerable reduction in number and size of dendritic spines. Ttyh1 transfected neurons had varicose swelling of dendrites and more stubby and mushroom spines. CONCLUSIONS: On the basis of our results we conclude that Ttyh1 may participate in regulation of dendritic spine formation. Ttyh1 protein does not affect on dendritic complexity. Formation of dendritic beading may be dependent on Na2+ and Cl- intracellular movement, followed by water movement to maintain osmolarity.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.45-46

Twórcy

autor

Gorniak-Walas M.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Kaliszewska A.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Lukasiuk K.

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland