Effect of salicylic acid potentiates cadmium-induced oxidative damage in Oryza sativa L. leaves

• Autorzy: Panda S.K. , Patra H. K.
• Języki publikacji: EN

Abstrakty

EN

In the present investigation, we studied the possible potentiating effect of salicylic acid (SA) under Cd toxicity in Oryza sativa L. leaves. Cd treatments for 24 h reduced the shoot length, dry biomass and total chlorophyll content followed by high Cd accumulation in shoots. About 16 h presoaking with SA resulted in partial protection against Cd, as observed by minor changes in length, biomass and total chlorophyll. SA priming resulted in low Cd accumulation. Enhanced thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H₂O₂) and superoxide anion (O₂⁻) content were seen when Cd was applied alone, while under SA priming the extent of TBARS, H₂O₂ and O₂⁻ were significantly low, suggesting SA-regulated protection against oxidative stress. The antioxidant enzymes like Catalase (CAT), guaiacol peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD) showed varied activities under Cd alone. CAT activity increased after Cd treatment, followed by a decline in GPX and GR activity. SOD also declined at the highest concentrations with an initial increase. Under SA-priming conditions, the efficiency of the antioxidant enzymes was significantly elevated. GPx and SOD activity showed significant increase in activity. The ascorbate activity increased after Cd treatment, followed by a decline in glutathione under SA-free condition. SA priming showed gradual increase in these non-enzymic antioxidants. Our results indicate that Cd-induced oxidative stress can be regulated by SA.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2007
• Tom: 29
• Numer: 6
• Opis fizyczny: p.567-575,fig.,ref.

Twórcy

autor

Panda S.K.

• Plant Biochemistry Laboratory, School of Life Sciences, Assam (Central) University, Silchar 788011, India

autor

Patra H. K.

• Post Graduate Department of Botany, Utkal Univerity, Bhubaneswar 751004, Orissa, India

Bibliografia

• Aidid SB, Okamoto B (1992) Effect of lead, cadmium and zinc on electric membrane potential at xylem symplast interface and cell elongation of Impatiens Balsamina. Environ Exp Bot 32:439–448
• Alscher RG, Donahue JL, Cramer CL (1997) Reactive oxygen species and antioxidants: relationship in green cells. Physiol Plant 100:224–233
• Alverez AL (2000) Salicylic acid in machinery of hypersensitive cell death and disease resistance. Plant Mol Biol 44:429–442
• Arnon DI (1949) Copper enzyme in the isolated chloroplast. Polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15
• Asada K (1994) Production and action of active oxygen species in photosynthetic tissues. In: Foyer CH, Mullineaux PM (eds) Causes of photooxidative stress and amelioration of defense system in plants. CRC, Boca Ration, FL, pp 77–104
• Asada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond CJ, Artzen CJ (eds) Photoinhibition: topics in photosynthesis. Elsevier, Amsterdam, pp 277–287
• Atal N, Saradhi PP, Mohanty P (1991) Inhibition of chloroplast photochemical reaction by treatments of wheat seedlings with low concentration of cadmium: analysis of electron transport activities and changes in fluorescence yield. Plant Cell Physiol 32:943–951
• Barceló J, Poschenrieder C (1990) Plant water relation as affected by heavy metal stress: a review. J Plant Nutr 13:1–37
• Bazzaz FA, Rolfe GL, Carlson RW (1992) Effect of cadmium on photosynthesis and transpiration of excised leaves of corn and sunflower. Physiol Plant 32:373–377
• Berukova MV, Sakhabutdinova R, Fatkhutdinova RA, Kyldiarova I, Shakirova F (2001) The role of hormonal changes in protective action of salicylic acid on growth of wheat seedlings under water deficit. Agrochemiya (Russ.) 2:51–54
• Chance B, Maehly AC (1955) Assay of catalase and peroxidase. Method Enzymol 2:746–778
• Chaoui A, Mazhoudi SS, Ghorbal MH, Ferjani EEL (1997) Cadmium and zinc induction of lipid peroxidation and effects the antioxidant enzyme activities in bean (Phaseolus vulgaris L). Plant Sci 127:139–147
• Chen CT, Chen TH, Lo KF, Chiu CY (2004) Effect of proline on copper transport in rice seedling under excess copper stress. Plant Sci 166:103–111
• Choudhury S, Panda SK (2004) Induction of oxidative stress and ultrastructural changes in moss Taxithelium nepalense (Schwaegr.) Broth. Under lead (Pb) and arsenic (As) phytotoxicity. Curr Sci (in press)
• Dat JF, Lopez-Delgado H, Foyer CH, Scott IM (1998) Parallel changes in H₂O₂ and catalase during the thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings. Plant Physiol 116:1351–1375
• De Vos CHR, Schat H (1991) Free radical and heavy metal tolerance. In: Rozema J, Verkleij JAC (eds) Ecological responses to environmental stresses. Kluwer, Dordrecht, pp 1–30
• Dietz KJ, Baier M, Kramer U (1999) Free radicals and reactive oxygen species as mediator of heavy metal toxicity in plants. In: Prasad MNV, Hagemeyer J (eds) Heavy metal stress in plants: from molecules to ecosystem. Springer, Berlin, pp 73–79
• Elstner EF (1982) Oxygen activation and oxygen toxicity. Annu Rev Plant
• Elstner EF, Heupel A (1976) Inhibition of nitrate formation from hydroxyl ammonium chloride: a simple assay for superoxide dismutase. Anal Biochem 70:616–620
• Enyedi AJ, Yalpani N, Sliverman P, Raskin I (1992) Signal molecule in systemic plant resistance to pathogens and pests. Cell 70:879–886
• Gallego SM, Benavioes MP, Tomaro ML (1996) Effect of heavy metal ions on sunflower leaves-evidence for involvement of oxidative stress. Plant Sci 121:151–159
• Giannopolitis CN, Ries SK (1977) Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol 59:309–314
• Gille G, Singler K (1995) Oxidative stress in living cells. Folia Microbiol 2:131–152
• Greger ML, Kautskey T, Sandberg AC (1995) A tentative model of Cd uptake in Potamogeton petinatus in relation to salinity. Environ Exp Bot 35:215–225
• Griffith OW (1980) Determination of glutathione and glutathione disulphide using glutathione reductase and 2- vinyl pyridine. Anal Biochem 106:207–211
• Halliwell B, Gutteridge JMC (1988) Free radical in biology and medicine. Clarendon Press, Oxford, p 1
• Harnendez JA, Campillo A, Jimenz A, Alarcon TJ, Sevilla F (1999) Response of antioxidant system and leaf water relation to NaCl stress in pea plants. New Phytol 141:241–251
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplast. I Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Hendry GAF, Baker AJM, Ewart CF (1992) Cadmium tolerance and toxicity, oxygen radical processes and molecular damage in cadmium-tolerant and cadmium-sensitive clones of Holcus lanatus. Acta Bot Neerl 41:271–281
• Himly AM, Sabana MB, Oaabees AY (1985) Bioaccumulation of cadmium: toxicity in Megul cephalus. Comp Biochem Physiol 81:139–140
• Janda T, Szalai G, Tari I, Paldi E (1999) Hydrophonic treatment with salicylic acid decreases the effect of chilling in maize (Zea mays L.) plants. Planta 208:175–180
• Karatglis S, Moustakas M, Symeonidis L (1991) Effect of heavy metals on isoperoxidase of wheat. Biol Plant 33:3–9
• Khale H (1993) Response of root of trees to heavy metals. Environ Exp Bot 33:99–119
• Levine A, Tenhaken R, Dixon R, Lamb C (1994) H₂O₂ from the oxidative brust orchestrates the plant hypersensitive disease resistance response. Cell 79:583–593
• Light harvesting complex II in radish cotyledons. Physiol Plant 73:518–524
• Lozano-Rodriguez E, Hernandez LE, Bonay P, Carpena-Ruiz RO (1997) Distribution of Cd in shoot and root tissues of maize and pea plants: physiological disturbances. J Exp Bot 48:123–128
• Malik D, Sheoran IS, Singh R (1992) Carbon metabolism in leaves of cadmium treated wheat seedlings. Plant Physiol Biochem 30:223–229
• Matewally A, Finkemeir I, Georgi M, Dietz K-J (2003) Salicylic acid alleviates cadmium toxicity in barley seedlings. Plant Physiol 132:272–281
• Mendelssohn IA, Mckee KL, Kong T (2001) A comparison of physiological indicators of sub lethal cadmium stress in wetland plants. Environ Exp Bot 46:263–275
• Mishra A, Choudhuri MA (1999) Effect of salicylic acid on heavy metal induced membrane deterioration mediated by lipooxygenases in rice. Biol Plant 42:409–415
• Mishra A, Choudhuri MA (1996) Possible implication of heavy metals (Pb²⁺ and Hg²⁺) in the free radical-mediated membrane damage in two rice cultivars. Ind J Plant Physiol 1:43–47
• Monk LS, Fagersted KV, Crawford RMM (1989) Oxygen toxicity and superoxide dismutase as an antioxidant in physiological stress. Physiol Plant 76:456–459
• Oser BL (1979) Hawks physiological chemistry. McGraw-Hills, New York
• Panda SK (2003) Heavy metal phytotoxicity induces oxidative stress moss Taxithelium sp. Curr Sci 84:631–633
• Panda SK, Chaudhury I, Khan MH (2003a) Heavy metal induced lipid peroxidation and affects antioxidants in wheat leaves. Biol Plant 46:289–294
• Panda SK, Singha LB, Khan MH (2003b) Does aluminium phytotoxicity induce oxidative stress in green gram (Vigna radiate)? Bulg J Plant Physiol 29:77–86
• Panda SK, Khan MH (2003) Chromium phytotoxicity effects on the germination of green gram (Vigna radiata) seeds. J Phytol Res 15:225–228
• Patra JB, Panda B (1998) A comparison of biochemical responses to oxidative and metal stress in seedlings of barley Hordeum vulgarae L. Environ Pollut 101:99–105; Physiology 33:78–96
• Prasad KVSK, Pardha Saradhi P, Sharmila P (1999) Concerted actionantioxidant enzyme and curtailed growth under zinc toxicity in Brassica napus. Environ Exp Bot 42:1–10
• Raskin I, Ehmann A, Melander WR, Mecuse BJD (1987) Salicylic acid: a natural inducer of heat production of Arum lilies. Science 237:1601–1602
• Rennenberg H (1982) Glutathione metabolism and possible role in higher plants. Phytochemistry 28:2771–2781
• Sagisaka S (1976) The occurrence of peroxide in perennial plant Populus glerica. Plant Physiol 57:308–309
• Sandalio LM, Dalurzo HC, Gomez M, Romero-Puertas MC, Del Rio LA (2001) Cadmium induced changes in growth and oxidative metabolism of pea plants. J Exp Bot 52:2115–2126
• Schutzendubel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Goldbold DL, Polle A (2001) Cadmium induced changes in antioxidant systems, hydrogen peroxide content and differentiation in Scots pine roots. Plant Physiol 127:887–889
• Shakriova FM, Bezrukova MV (1997) Induction of wheat resistance against environmental salinization by salicylic acid. Biol Bull 24:109–112
• Shirasu K, Nakajima A, Rajshekar K, Dixon RA, Lamb C (1997) Salicylic acid potentiates an agonist-dependent gain control that amplifies pathogen signal in the activation of defence mechanism. Plant Cell 9:261–270
• Skorzynska-Polit E, Darzkiewicz M, Krupa Z (2004) The activity of antioxidant system in cadmium treated Arabidopsis thaliana. Biol Plant 47:71–78
• Smith IK, Vierhgeller TL, Throne CA (1988) Assay of glutathione reductase in crude tissue homogenate using 5, 5’, dithiobis (2 – nitrobenzoic acid). Anal Biochem 175:408–413
• Somashekaraiah BV, Padmaja K, Prasad ARK (1992) Phytotoxicity of cadmium ions on germinating seedlings of mung beans (Phaseolus vulgaris): involvement of lipid peroxides in chlorophyll degradation. Physiol Plant 85:85–89
• Verma S, Dubey RS (2003) Lead toxicity indices lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci 164:645–655
• Yang MN, Wang J, Wang SH, Xu LL (2003) Salicylic acid induced aluminium tolerance by modulation of citrate efflux from roots of Cassia tora L. Planta 217(1):168–174