Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

• Autorzy: Sliwinska A.A. , Syklowska-Baranek K. , Kosmider A. , Granica S. , Miszczak K. , Nowicka G. , Kasztelan A. , Podsadni P. , Turlo J. , Pietrosiuk A.
• Języki publikacji: EN

Abstrakty

EN

In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of Polyscias filicifolia was developed. The phenolic compound content in P. filicifolia has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 μM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of P. filicifolia extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed. The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 μM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 μM SA and 200 μM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2018
• Tom: 87
• Numer: 2
• Opis fizyczny: Article 3586 [16p.],fig.,ref.

Twórcy

autor

Sliwinska A.A.

• Department of Pharmaceutical Biology and Medicinal Plant Biotechnology, Faculty of Pharmacy with Division of Laboratory Medicine, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Syklowska-Baranek K.

• Department of Pharmaceutical Biology and Medicinal Plant Biotechnology, Faculty of Pharmacy with Division of Laboratory Medicine, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Kosmider A.

• Department of Biochemistry and Pharmacogenomics, Faculty of Pharmacy with Division of Laboratory Medicine and Center for Preclinical Studies, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Granica S.

• Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy with Division of Laboratory Medicine, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Miszczak K.

• Department of Pharmaceutical Biology and Medicinal Plant Biotechnology, Faculty of Pharmacy with Division of Laboratory Medicine, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Nowicka G.

• Department of Biochemistry and Pharmacogenomics, Faculty of Pharmacy with Division of Laboratory Medicine and Center for Preclinical Studies, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Kasztelan A.

• Department of Pharmaceutical Biology and Medicinal Plant Biotechnology, Faculty of Pharmacy with Division of Laboratory Medicine, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Podsadni P.

• Department of Drug Technology and Pharmaceutical Biotechnology, Faculty of Pharmacy with Division of Laboratory Medicine, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Turlo J.

• Department of Drug Technology and Pharmaceutical Biotechnology, Faculty of Pharmacy with Division of Laboratory Medicine, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

autor

Pietrosiuk A.

• Department of Pharmaceutical Biology and Medicinal Plant Biotechnology, Faculty of Pharmacy with Division of Laboratory Medicine, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland

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Identyfikatory

DOI

10.5586/asbp.3586