Impact of Panax ginseng and Ginkgo biloba extracts on expression level of transcriptional factors and xenobiotic-metabolizing cytochrome P450 enzymes

• Autorzy: Bogacz A. , Karasiewicz M. , Dziekan K. , Procyk D. , Gorska-Paukszta M. , Kowalska A. , Mikolajczak P.L. , Ozarowski M. , Czerny B.

Warianty tytułu

PL

Wpływ ekstraktów z Panax ginseng i Ginkgo biloba na poziom ekspresji czynników transkrypcyjnych i enzymów cytochromu P450 metabolizujących ksenobiotyki

• Języki publikacji: EN

Abstrakty

EN

Introduction: Despite widespread use of Panax ginseng and Ginkgo biloba, the data on the safety as well as herb-drug interactions are very limited. Therefore, we postulate that P. ginseng and G. biloba may modulate the activity and content of cytochrome P450 isozymes involved in the biotransformation of diverse xenobiotic substances. Objective: The aim of our study was to determine the influence of herbal remedies on the expression level of CYP enzymes and transcriptional factors. Methods: Male Wistar rats were given standardized Panax ginseng (30 mg/kg p.o.) or standardized Ginkgo biloba (200 mg/kg p.o.) for 3 and 10 days. The expression in liver tissue was analyzed by realtime PCR method. Results: Our results showed a decrease of CYP3A1 (homologue to human CYP3A4) mRNA level after P. ginseng extract treatment. The CYP2C6 (homologue to human CYP2C9) expression was also reduced. Additionally, after 10 days of the treatment with P. ginseng an increase of CYP1A1 (homologue to human CYP1A1) and CYP1A2 (homologue to human CYP1A2) expression was observed. Moreover, G. biloba extract also caused an increase of expression level for CYP1A1, CYP2C6, CYP3A1 and CYP3A2. Conclusion: Our findings suggest that herbal extracts can modulate the expression of transcriptional factors and CYP enzymes involved in xenobiotic metabolism and chemical carcinogenesis.

PL

Wstęp: Mimo powszechnego stosowania Panax ginseng i Ginkgo biloba dane dotyczące bezpieczeństwa, a także interakcji pomiędzy preparatami roślinnymi a lekami syntetycznymi są bardzo ograniczone. W niniejszych badaniach założono, iż żeń-szeń oraz miłorząb mogą modulować aktywność i zawartość izoenzymów cytochromu P450 biorących udział w biotransformacji różnych substancji ksenobiotycznych. Cel: Określenie wpływu preparatów roślinnych na poziom ekspresji enzymów CYP i ich czynników transkrypcyjnych. Metody: Szczurom rasy Wistar podawano standaryzowany Panax ginseng (30 mg/kg) oraz Ginkgo biloba (200 mg/kg) przez 3 do 10 dni. Ekspresję w tkance wątrobowej analizowano za pomocą metody PCR w czasie rzeczywistym. Wyniki: Uzyskane wyniki wykazały spadek poziomu mRNA CYP3A1 (homolog ludzkiego enzymu CYP3A4) po podaniu ekstraktu z żeń-szenia. Ekspresja genu CYP2C6 (homolog ludzkiego enzymu CYP2C9) również uległa obniżeniu. Dodatkowo, obserwowaliśmy wzrost ekspresji CYP1A1 (homolog ludzkiego enzymu CYP1A1) i CYP1A2 (homolog ludzkiego enzymu CYP1A2) po 10 dniach stosowania P. ginseng. Ponadto, ekstrakt z G. biloba spowodował również wzrost poziomu mRNA CYP1A1, CYP2C6, CYP3A1 i CYP3A2 w modelu in vivo. Wnioski: Badania sugerują, że wyciągi roślinne mogą modulować ekspresję czynników transkrypcyjnych i enzymów CYP uczestniczących w metabolizmie ksenobiotyków i chemicznej karcynogenezie.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2016
• Tom: 62
• Numer: 1
• Opis fizyczny: p.42-54,fig.,ref.

Twórcy

autor

Bogacz A.

• Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Karasiewicz M.

• Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland

autor

Dziekan K.

• Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland

autor

Procyk D.

• Department of Laboratory Diagnostics, Greater Poland Cancer Centre, Garbary 15, 61-866 Poznan, Poland

autor

Gorska-Paukszta M.

• Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland

autor

Kowalska A.

• Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland

autor

Mikolajczak P.L.

• Department of Pharmacology, Poznan University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland
• Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland

autor

Ozarowski M.

• Department of Pharmacology and Phytochemistry, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
• Departament of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Czerny B.

• Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
• Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Rybacka 1, 70-204 Szczecin, Poland

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Identyfikatory

DOI

10.1515/hepo-2016-0004