Antioxidant, anticancer and hepatoprotective activities of Cotoneaster horizontalis Decne extract as well as a-tocopherol and amygdalin production from in vitro culture

• Autorzy: Sokkar N. , El-Gindi O. , Sayed S. , Mohamed S. , Ali Z. , Alfishawy I.
• Języki publikacji: EN

Abstrakty

EN

The phytochemical analysis of the ethanolic extract of branches of Cotoneaster horizontalis, Decne revealed the presence of: β-carotene, ascorbic acid and less amounts of α-tocopherol and amygdalin (vitamin B17) in proportions of: 2,500, 70, 0.093, 0.334 mg 100 g-1, respectively. Acute oral toxicity test revealed its safety profile. In vitro study revealed its good 2, 2-diphenyl-1- picrylhydrazyl radical scavenging and anticancer activities. Invivo study, simultaneous administration of this extract at a dose of 100 or 200 mg kg-1 body weight for 4 weeks, exhibited a significant protection in a dose-dependant manner against hepatotoxicity induced by repeated dose of acetaminophen (1 g kg-1 body weight day-1, p.o.) by preserving the liver function parameters, hepatic redox state and serum lipid profile near the healthy levels. Consequently, in vitro culture was carried out on full or half strength of Murashige and Skoog medium supplemented with different concentrations of benzyl amino purine or kinetin provided shootlets production; different concentrations of 2,4-dichlorophenoxy acetic acid and naphthalene acetic acid showed an increase of callus. Determination of a-tocopherol and amygdalin in different shootlets and callus extracts showed a pronounced increases up to 30.62 and 3.69 mg 100 g-1 in shootlet extract, respectively as well as 26.61 and 12.71 mg 100 g-1 in callus extract, respectively, as compared with those of the mother plant (0.76 and 0.11 mg 100 g-1 extract, respectively).

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 08
• Opis fizyczny: p.2421-2428,fig.,ref.

Twórcy

autor

Sokkar N.

• Natural Products and Alternative Medicine Department, Faculty of Pharmacy, King Abdulaziz University, Jeddah 80200, Kingdom of Saudi Arabia

autor

El-Gindi O.

• Pharmacognosy Department, Faculty of Pharmacy, Russian Univ. Cairo, Cairo 11829, Egypt

autor

Sayed S.

• Ornamental Department, Horticulture Research Institute, Agricultural Research Center, Giza 12619, Egypt

autor

Mohamed S.

• Pharmacognosy Department, Faculty of Pharmacy, AL-Azhr Univ, Cairo 11884, Egypt

autor

Ali Z.

• Biochemistry Department, National Organization for Drug Control and Research (NODCAR), Giza 354, Egypt

autor

Alfishawy I.

• Medical Administration, Al-Azhar Univ, Cairo 11884, Egypt

Bibliografia

• Bhaumik A, Sharma MC (2002) Therapeutic efficacy of Liv.52 in paracetamol induced hepatopathy in rabbits. Indian J Vet Res 1:8–17
• Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 28:25–30
• Brent J (2005) Critical care toxicology: diagnosis and management of critically poisoned patient. Elsevier Health Sciences, Amazon.com
• Cakilcioglu U, Turkoglu I (2010) An ethnobotanical survey of medicinal plants in Sivrice (Elazıg-Turkey). J Ethnopharmacol 132:165–175
• Chin JH, Hussin AH, Ismail S (2009) Anti-hepatotoxicity effect of Orthosiphon stamineus Benth against acetaminophen-induced liver injury in rats by enhancing hepatic GST activity. Phcog Res 1:53–58
• Davern TJ, James LP, Hinson JA, Polson J, Larson AM, Fontana RJ, Lalani E, Munoz S, Shakil AO, Lee WM (2006) Measurement of serum acetaminophen-protein adducts in patients with acute liver failure. Gastroenterology 130:687–694
• Du Q, Jerz G, Ha Y, Li L, Xu Y, Zhang Q, Zheng Q, Winterhalter P, Ito Y (2005) Semi-industrial isolation of salicin and amygdalin from plant extracts using slow rotary counter-current chromatography. J Chromatogr A 1074:43–46
• El-Mousallamy AM, Hussein SA, Irmgard M, Nawwar MA (2000) Unusual phenolic glycosides from Cotoneaster orbicularis. Phytochemistry 53:699–704
• Escriva A, Esteve MJ (2002) Determination of liposoluble vitamins in cooked meals, milk and milk products by liquid chromatography. J Chromatogr A 947:313–318
• Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499–505
• Kapche GD, Harmut L, Serge F, Simeon FK, Pascal W, Bonaventure TN, Berhanu MA (2007) Lanneanol: a new cytotoxic dihydroalkylcyclohexenol and phenolic compounds from Lannea nigritana (Sc. Ell.) Keay. Indian J Vet Res 35:539–543
• Khalifa SF, Loutify MH (2006) Ornamental cultured plant collection. In: The occasion of the first international conference on: strategy of Botanic Gardens, vol 31
• Konings EJM, Roomans HHS (1996) Evaluation and validation of an LC method for the analysis of carotenoids in vegetables and fruits. J Food Chem 59:599–603
• Kulkarni KS, Rafiq M, Gopumadhavan S, Venkataranganna MV, Madhumathi BG, Mitra SK (2002) Protective effect of Liv. 52 on Na+-K+-ATPase activity in paracetamol-induced hepatotoxicity. Med Update 5:53–56
• Mohit D, Parminder N, Jaspreet N, Manish M (2011) Hepatotoxicity V/S hepatoprotective agents-a pharmacological review. Int Res J Pharm 2(3):31–37
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
• Neilsen SS (1998) Food analysis, 2nd edn. Aspen Publishers, Inc, Gaitherburg, pp 287–288
• OECD (1996) Guidelines for the testing of chemicals. Test no. 423: acute oral toxicity-acute toxic class method. Organisation for economic co-operation and development, Paris
• Palme E, Bilia AR, Morelli I (1996) Flavonols and isoflavones from Cotoneaster simonsii. Phytochemistry 42(3):903–905
• Raghavendran HR, Sathivel A, Devaki T (2005) Effect of Sargassum polycystum (Phaeophyceae)-sulphated polysaccharide extract against acetaminophen-induced hyperlipidemia during toxic hepatitis in experimental rats. Mol Cell Biochem 276:89–96
• Rajneesh CP, Manimaran A, Sasikala K R, Adaikappan P (2008) Lipid peroxidation and antioxidant status in patients with breast cancer. Singapore Med J 49(8): 640–463
• Skehan P, Storeng R (1990) New coloremtric cytotoxicity assay for anti-cancer drug screening. J Natl Cancer Inst 82:1107–1112
• Wang B, Ji C (2008) Tannin concentration enhances seed caching by scatter-hoarding rodents: an experiment using artificial seeds. Acta Oecol 34:379–385
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