Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19

• Autorzy: Uslu A. , Sanioglu Golen G. , Agah Tekindal M. , Sakmanoglu A. , Sayın Z. , Denizli O. , Gok A. , Erganis O.
• Języki publikacji: EN

Abstrakty

EN

Brucellosis is a worldwide zoonosis, that can still be classified as endemic despite its ancient origins which causes economic losses and public health problems. Although effectively controlled by vaccination in animals, there is currently no vaccine for use in humans. Outer Membrane Proteins (OMP) that play an active immunogenic and protective role in the Brucellae family. OMP19 is present in all Brucella species as a surface antigen and is a potent immunogen responsible for Brucellosis intracellular infection. For this reason, the study was aimed to be used safely as a potential recombinant vaccine candidate against all Brucella infections, especially in humans and pregnant animals. This study evaluated a Brucella lipoprotein antigen, i.e. 19 kilodalton (kDa) outer membrane protein (OMP19), which was amplified and cloned into the pETSUMO vector system. The immunogenic power of the purified recombinant OMP19 antigen against brucellosis was compared with that of OMP19 (Raybiotech Inc, USA) in a mouse model and the obtained rOMP19 antigen was found to be similar to the commercially available recombinant protein.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2022
• Tom: 25
• Numer: 4
• Opis fizyczny: p.561-569,fig.,ref.

Twórcy

autor

Uslu A.

• Microbiology Department, Faculty of Veterinary Medicine, Selcuk University, Alaeddin Keykubat Campus, Selcuklu, Konya, 42130, Turkey

autor

Sanioglu Golen G.

• Microbiology Department, Faculty of Veterinary Medicine, Aksaray University, Aksaray, 68100, Turkey

autor

Agah Tekindal M.

• Biostatistics Department, Faculty of Medicine, Izmir Katip Çelebi University, Cigli Campus, Cigli, Izmir, 35620, Turkey

autor

Sakmanoglu A.

• Microbiology Department, Faculty of Veterinary Medicine, Selcuk University, Alaeddin Keykubat Campus, Selcuklu, Konya, 42130, Turkey

autor

Sayın Z.

• Microbiology Department, Faculty of Veterinary Medicine, Selcuk University, Alaeddin Keykubat Campus, Selcuklu, Konya, 42130, Turkey

autor

Denizli O.

• Microbiology Department, Faculty of Veterinary Medicine, Selcuk University, Alaeddin Keykubat Campus, Selcuklu, Konya, 42130, Turkey

autor

Gok A.

• Microbiology Department, Faculty of Veterinary Medicine, Selcuk University, Alaeddin Keykubat Campus, Selcuklu, Konya, 42130, Turkey

autor

Erganis O.

• Microbiology Department, Faculty of Veterinary Medicine, Selcuk University, Alaeddin Keykubat Campus, Selcuklu, Konya, 42130, Turkey

Identyfikatory

DOI

10.24425/pjvs.2022.143539