Pilocarpine-induced status epilepticus alters hippocampal PKC expression in mice

• Autorzy: Liu J.X. , Liu Y. , Tang F. R.
• Języki publikacji: EN

Abstrakty

EN

We investigated the protein expression of different protein kinase C (PKC) isoforms (PKCa, PKCpi, PKCp2, PKCy, PKC5, PKCe, PKCn and PKCQ in the hippocampus of normal control mice and progressive changes in PKC isoforms expression during and after pilocarpine induced status epilepticus (PISE). We showed the reduced expression of PKC5, PKCn and PKCÇ in interneurons in the CA1 area and in the hilus of the dentate gyrus during or after PISE. Increased expression of PKCa and PKCpi was demonstrated in the stratum pyramidale of CA3 area, and PKCe was up-regulated in the stratum lucidum of the CA3 area during or after PISE. Our results suggest that hippocampal PKC isoforms may play different roles in seizure generation, and be targets for development of anti-convulsive drugs.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: 2
• Opis fizyczny: p.220-232,fig.,ref.

Twórcy

autor

Liu J.X.

• Institute of Neurobiology, School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China

autor

Liu Y.

• Institute of Neurobiology, School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China

autor

Tang F. R.

• Temasek Laboratories, National University of Singapore, Singapore, Sigapore

Bibliografia

• Bich-Hoai TT, Marin A, Dinu C, Banciu D, Maria-Luiza F, Ristoiu V (2010) Hypoxia and high glucose activate tet- rodotoxin-resistant Na(+) currents through PKA and PKC. Acta Neurobiol Exp (Wars) 70: 351-361.
• Coffey ET, Sihra, TS, Nicholls, DG (1993) Protein kinase C and the regulation of glutamate exocytosis from cerebro- cortical synaptosomes. J Biol Chem 268: 21060-21065.
• Crespel A, Coubes P, Rousset MC, Brana C, Rougier A, Rondouin G, Bockaert J, Baldy-Moulinier M, Lerner- Natoli M (2002) Inflammatory reactions in human medial temporal lobe epilepsy with hippocampal sclerosis. Brain Res 952: 159-169.
• Cuellar JC, Griffith EL, Merlin LR (2005) Contrasting roles of protein kinase C in induction versus suppression of group I mGluR-mediated epileptogenesis in vitro. J Neurophysiol 94: 3643-3647.
• Daigen A, Akiyama K, Otsuki S (1991) Long-lasting change in the membrane-associated protein kinase C activity in the hippocampal kindled rat. Brain Res 545: 131-136.
• Fuortes MG, Faria LC, Merlin LR (2008) Impact of protein kinase C activation on epileptiform activity in the hip- pocampal slice. Epilepsy Res 82: 38-45.
• Grabauskas G, Chapman H, Wheal HV (2006) Role of pro­tein kinase C in modulation of excitability of CA1 pyra­midal neurons in the rat. Neuroscience 139: 1301-1313.
• Guglielmetti F, Rattray M, Baldessari S, Butelli E, Samanin R, Bendotti C (1997) Selective up-regulation of protein kinase C epsilon in granule cells after kainic acid-induced seizures in rat. Brain Res Mol Brain Res 49:188-196.
• Kohira I, Akiyama K, Daigen A, Otsuki S (1992) Enduring increase in membrane-associated protein kinase C activ­ity in the hippocampal-kindled rat. Brain Res 593: 82-88.
• Liu JX, Cao X, Tang YC, Liu Y, Tang FR (2007) CCR7, CCR8, CCR9 and CCR10 in the mouse hippocampal CA1 area and the dentate gyrus during and after pilo- carpine-induced status epilepticus. J Neurochem 100: 1072-1088.
• Liu JX, Tang YC, Liu Y, Tang FR (2008) mGluR5-PLCbeta4- PKCbeta2/PKCgamma pathways in hippocampal CA1 pyramidal neurons in pilocarpine model of status epilepti- cus in mGluR5+/+ mice. Epilepsy Res 82: 111-123.
• Madison DV, Malenka RC, Nicoll RA (1986) Phorbol esters block a voltage-sensitive chloride current in hippocampal pyramidal cells. Nature 32: 695-697.
• McNamara RK, Wees EA, Lenox RH (1999) Differential subcellular redistribution of protein kinase C isozymes in the rat hippocampus induced by kainic acid. J Neurochem 72: 1735-1743.
• Niimura M, Moussa R, Bissoon N, Ikeda-Douglas C, Milgram NW, Gurd JW (2005) Changes in phosphorylation of the NMDA receptor in the rat hippocampus induced by status epilepticus. J Neurochem 92: 1377-1385.
• Ono M, Akiyama K, Tsutsui K, Kuroda S (1994) Differential changes in the activities of multiple protein kinase C sub­species in the hippocampal-kindled rat. Brain Res 660: 27-33.
• Prekeris R, Mayhew MW, Cooper JB, Terrian DM (1996) Identification and localization of an actin-binding motif that is unique to the epsilon isoform of protein kinase C and participates in the regulation of synaptic function. J Cell Biol 132: 77-90.
• Represa A, Niquet J, Pollard H, Ben-Ari Y (1995) Cell death, gliosis, and synaptic remodeling in the hippocam­pus of epileptic rats. J Neurobiol 26: 413-425.
• Silva AP, Lourengo J, Xapelli S, Ferreira R, Kristiansen H, Woldbye DP, Oliveira CR, Malva JO (2007) Protein kinase C activity blocks neuropeptide Y-mediated inhibition of glutamate release and contributes to excitability of the hip­pocampus in status epilepticus. FASEB J 21: 671-681.
• Tang FR, Lee WL, Gao H, Chen Y, Loh YT, Chia SC (2004) Expression of different isoforms of protein kinase C in the rat hippocampus after pilocarpine-induced status epi- lepticus with special reference to CA1 area and the den­tate gyrus. Hippocampus 14: 87-98.
• Terunuma M, Xu J, Vithlani M, Sieghart W, Kittler J, Pangalos M, Haydon PG, Coulter DA, Moss SJ (2008) Deficits in phosphorylation of GABA(A) receptors by intimately associated protein kinase C activity underlie compromised synaptic inhibition during status epilepti­cus. J Neurosci 28: 376-384.
• Usachev YM, Marsh AJ, Johanns TM, Lemke MM, Thayer SA (2006) Activation of protein kinase C in sensory neu­rons accelerates Ca2+ uptake into the endoplasmic reticu­lum. J Neurosci 26: 311-318.

Uwagi

Rekord w opracowaniu