Effects of D1 receptor agonist on voltage-gated sodium currents in mPFC pyramidal neurons in rats of different ages

• Autorzy: Szulczyk B. , Grzelka K.
• Języki publikacji: EN

Abstrakty

EN

We recorded voltage-gated Na+ currents from mPFC pyramidal neurons in slices. The recordings were made in cell-attached configuration (macropatches) in young (3-weeks-old) and adult (9-weeks-old) rats. Fast synaptic transmission was blocked. The maximal Na+ currents amplitude varied in different recordings because of different number of channels in different patches. The mean amplitude of Na+ currents was 16.7 ± 1.4 (n=45) in young rats and 8.4 ± 1.2 pA (n=45) in adult rats (P<0.001). Sodium currents of big amplitude were selected to test the effects of a D1 receptor agonist (SKF 38393 10 µM). In young rats the maximal sodium current amplitude was 28.9 ± 3.7 pA in control and 29.7 ± 3.8 pA in the presence of SKF 38393 (P>0.05, n=7). Moreover in young rats the potential of half-maximal activation (V0.5) was not significantly different in control and with SKF 38393 in the bath (−11.5 ± 1.9 mV and −14.7 ± 1.2 mV respectively, n=7, P>0.05).In adult rats the maximal sodium current amplitude was 33.5 ± 10 pA and 32.5 ± 8.9 pA in control and in the presence of SKF 38393 respectively (P>0.05, n=5). Also in adult rats the potential of halfmaximal activation was −11.9 ± 1.6 mV in control and −14.2 ± 1.8 mV with SKF 38393 in the bath (n=5, P<0.05). We suggest that D1 receptor agonist does not influence maximal Na+ currents and exerts small effects on their kinetic properties in rats of different ages. Supported by grant no. NCN/MNiSzW- N N401 030037.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2012
• Tom: 72
• Numer: 2
• Opis fizyczny: p.199

Twórcy

autor

Szulczyk B.

• Department of Physiology and Pathophysiology, Medical University of Warsaw, Warsaw, Poland

autor

Grzelka K.

• Department of Physiology and Pathophysiology, Medical University of Warsaw, Warsaw, Poland

Uwagi

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