Gene expression of RNAP II, JBP1 and JBP2 in Leishmania major exposed to antimonials, amphotericin B and paromomycin

• Autorzy: Ajamein V. , Eslami G. , Ahmadian S. , Khamesipour A. , Elloumi M.
• Języki publikacji: EN

Abstrakty

EN

Cutaneous leishmaniosis (CL) is treated with pentavalent antimony (SbV) as a first-line drug, while amphotericin B and paromomycin are potential alternatives in antimonial- resistant isolates. However, the mechanisms of drug resistance remain unclear. The present study analyses the gene expression of RNA polymerase II (RNAP II) and J-binding protein 1 (JBP1), and J-binding protein 2 (JBP2) in Leishmania major after exposure to drugs in vitro. L. major (MRHO/IR/75/ER) promastigotes were exposed to various concentrations of glucantime, paromomycin and amphotericin B for 72 hours. The RNA was then extracted and used for cDNA synthesis. The expressions of JBP1, JBP2 and RNAP II were analysed using SYBR Green real-time PCR. No change in JBP2 or RNAP II expression was associated with amphotericin B, but JBP1 expression decreased with increasing drug concentration. Paromomycin had no effect on JBP2 expression, but a 13.5-fold increase in JBP1 was observed at 100 μg/ml, and a decrease in RNAP II expression at 25 and 50 μg/ml. Exposure to glucantime resulted in 1.4-fold lower JBP1 expression at 5 μg/ml, and 333.33- to 500-fold lower RNAP II at concentrations of 5 to 15 μg/ml. As Base J synthesis requires both JBP1 and JBP2, RNAP II (encoding RNA polymerase II) could reduce expression. However, RNAP II was not expressed in all groups, indicating that the genes associated with drug resistance may be regulated in other ways.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2018
• Tom: 64
• Numer: 3
• Opis fizyczny: p.181-187,fig.,ref.

Twórcy

autor

Ajamein V.

• Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blv., 8916188638 Yazd, Iran
• Department of Parasitology and Mycology, Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blv., 8916188638 Yazd, Iran

autor

Eslami G.

• Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blv., 8916188638 Yazd, Iran
• Department of Parasitology and Mycology, Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blv., 8916188638 Yazd, Iran

autor

Ahmadian S.

• Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blv., 8916188638 Yazd, Iran
• Department of Parasitology and Mycology, Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blv., 8916188638 Yazd, Iran

autor

Khamesipour A.

• Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Taleghani, 8916188638 Tehran, Iran

autor

Elloumi M.

• Laboratory of Technologies of Information and Communication and Electrical Engineering (LaTICE), University of Tunis, 5 Avenue Taha Hussein, B.P. 56, Bab Menara, Tunis, Tunisia

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Identyfikatory

DOI

10.17420/ap6403.149