1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5

• Autorzy: Dabrowski S. , Pietrewicz-Kubicz E. , Zablotna E. , Dlugolecka A.
• Języki publikacji: EN

Abstrakty

EN

 1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.

Słowa kluczowe

EN

1,3-propanediol; production; Escherichia coli; gene expression; dha operon; Clostridium butyricum; anaerobic fermentation; biodiesel fuel

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2012
• Tom: 59
• Numer: 3
• Opis fizyczny: p.357-361,fig.,ref.

Twórcy

autor

Dabrowski S.

• A and A Biotechnology, Gdynia, Poland

autor

Pietrewicz-Kubicz E.

autor

Zablotna E.

autor

Dlugolecka A.

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