Partial characterization and antimicrobial activity of peptides from Amburana cearensis seeds against phytopathogenic fungi and yeasts

• Autorzy: dos Santos P.H.A. , dos Santos I. S. , Melo V. M. M. , Vasconcelos I. M. , Carvalho A. d. O. , Gomes V. M.
• Języki publikacji: EN

Abstrakty

EN

The aim of this work was to study the antifungal action of a protein and peptide-rich fraction from Amburana cearensis seeds. Initially, proteins were extracted from seed flour in phosphate buffer and re-extracted with 1 M lithium chloride. The products obtained from both extractions were precipitated with ammonium sulfate at 70% saturation, and the precipitates were re-suspended in distilled water, heated at 80℃ for 15 min and clarified by centrifugation at 12,000×g. The obtained fractions from both extractions were dialyzed, recovered by lyophilization and visualized in Tris–Tricine/SDS gel electrophoresis. The fractions obtained were rich in proteins of low molecular weight and were submitted to antifungal assays. Fraction S4 (extraction with phosphate buffer) inhibited the fungi, Colletotrichum lindemuthianum, Fusarium oxysporum, Fusarium solani, Candida albicans and Saccharomyces cerevisiae. Fractions P2 (re-extraction with lithium chloride) had a low or non-inhibitory effect on the fungi tested.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 3
• Opis fizyczny: p.597-603,fig.,ref.

Twórcy

autor

dos Santos P.H.A.

• Laborato´rio de Fisiologia e Bioquı´mica de Microrganismos, Universidade Estadual do Norte Fluminense, Campos, RJ 28013-602, Brazil

autor

dos Santos I. S.

• Laborato´rio de Fisiologia e Bioquı´mica de Microrganismos, Universidade Estadual do Norte Fluminense, Campos, RJ 28013-602, Brazil

autor

Melo V. M. M.

• Departamento de Biologia, Universidade Federal do Ceara´, Fortaleza, CE 60451-970, Brazil

autor

Vasconcelos I. M.

• Departamento de Bioquı´mica e Biologia Molecular, Universidade Federal do Ceara´, Fortaleza, CE 60451-970, Brazil

autor

Carvalho A. d. O.

• Laborato´rio de Fisiologia e Bioquı´mica de Microrganismos, Universidade Estadual do Norte Fluminense, Campos, RJ 28013-602, Brazil

autor

Gomes V. M.

• Laborato´rio de Fisiologia e Bioquı´mica de Microrganismos, Universidade Estadual do Norte Fluminense, Campos, RJ 28013-602, Brazil

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