Genotoxicity of soil pollutants extracted with different solvents

• Autorzy: Jadczyk P. , Kolwzan B.
• Języki publikacji: EN

Abstrakty

EN

The aim of our study was to compare the efficacy of extraction of genotoxic pollutants from soils performed by means of several solvents. Soil pollutants were extracted in the Soxhlet apparatus. Extract genotoxicity was assessed using the Ames Test. All the examined soils (12) contained genotoxic pollutants. Extraction with hexane allowed detection of pollution genotoxicity in 11 soils, whereas with methanol and dichloromethane in 10 soils each. The other solvents allowed detection of genotoxicity in fewer soil samples. Methanol extract had the highest genotoxicity in the majority of soils (7 out of 12). Results obtained in the present study and literature data allow recommendation of methanol for extraction of genotoxic pollutants from soils. Methanol is more versatile, efficient, common, and less harmful for laboratory workers than dichloromethane and hexane.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2013
• Tom: 22
• Numer: 1
• Opis fizyczny: p.141-147,ref.

Twórcy

autor

Jadczyk P.

• Institute of Environment Protection Engineering, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland

autor

Kolwzan B.

• Institute of Environment Protection Engineering, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland

Bibliografia

• 1. JADCZYK P., KOŁWZAN B. The application of the Ames test to the evaluation of the mutagenic activity of soil’s pollutants. Postępy Mikrobiologii, in press. [In Polish].
• 2. CHO B.-H., CHINO H., TSUJI H., UNITO T., NAGANKA K., OTSUKA S., YAMASHITA K., MATSUMOTO S., OYAIZU H. Laboratory-scale bioremediation of oil-contaminated soil of Kuwait with soil amendment materials. Chemosfere 35, 1599, 1997.
• 3. SATO S., MATSUMURA A., URUSHIGAWA Y. Type analysis and mutagenicity of petroleum oil extracted from sediment and soil samples In Kuwait. Environ. International 24, 67, 1998.
• 4. GEORGE S.E., HUGGINS-CLARK G., BROOKS L.R. Use of a Salmonella microsuspensions of munitions compounds at low concentrations. Mutat. Res. 490, 45, 2001.
• 5. KOŁWZAN B., JADCZYK P., RYBAK J., ADAMIAK W. Genotoxicity of explosives contaminated soil before and after bioremediation. Environ. Prot. Eng. 34, 53, 2008.
• 6. MATSUSHITA T., MATSUI Y., SAKUMA S., INOUE T. Changes in mutagenicity of herbicide chlornitrofen during biodegration. Mutat. Res. 516, 71, 2002.
• 7. MORELLI I.S., VECCHIOLI G.I., DEL PANNO M.T., PAINCERA M.T. Effect of petrochemical sludge concentrations on changes in mutagenic activity during soil bioremediation process. Environ. Toxicol. Chem. 20, 2179, 2001.
• 8. KOOL H.J., VAN KREYL C.F., PERSAD S. Mutagenic activity in groudwater in relation to mobilization of organic mutagens in soil. Sci. Total. Environ. 84, 185, 1989.
• 9. DONELLY K.C., BROWN K.W., ANDERSON C.S., THOMAS J.C., SCOTT B.R. Bacterial mutagenicity and acute toxicity of solvent and aqueous extracts of soil samples from a abandoned chemical manufacturing site. Environ. Toxicol. Chem. 10, 1123, 1991.
• 10. ALEEM A., MALIK A. Genotoxic hazards of long-term application of wastewater on agricultural soil. Mutat. Res. 538, 145, 2003.
• 11. BROWN K.W., DONELLY K.C., THOMAS J.C., DAVOL P., SCOTT B.R. Mutagenicity of 3 agricultural soils. Sci. Total. Environ. 41, 173, 1985.
• 12. KNIZE M.G., TAKEMOTO B., LEWIS P.R., FELTON J.S. The characterization of the mutagenic activity of soil. Mutat. Res. 192, 23, 1987.
• 13. WESP H.F., TANG X., EDENHARDER R. The influence of automobile exhausts on mutagenicity of soils: contamination with, fractionation, separation, and preliminary identification of mutagens in the Salmonella/reversion assay and effects of solvent fractions on the sister-chromatid exchanges in human lymphocyte cultures and in the in vivo mouse bone marrow micronucleus assay. Mutat. Res. 472, 1, 2000.
• 14. DA SILVA JÚNIOR F.M.R., VAZ ROCHA J.A., VARGAS V.M.F. Extraction parameters in the mutagenicity assay of soil samples. Sci. Total. Environ. 407, 6017, 2009.
• 15. COURTY B., LE CURIEUX F., MILON V., MARZIN D. Influence of extraction parameters on the mutagenicity of soil samples. Mutat. Res. 565, 23, 2004.
• 16. ANSARI M., MALIK A. Genotoxicity of agricultural soils in the vinicity of industrial area. Mutat. Res. 673, 124, 2009.
• 17. EDENHARDER R., ORTSEIFEN M., KOCH M., WESP H.F. Soil mutagens are airborne mutagens: variation of mutagenic activities induced in Salmonella typhimurium TA 98 and TA 100 by organic extracts of agricultural and forest soils in dependence on location and season. Mutat. Res. 472, 23, 2000.
• 18. MARON D.M., AMES B.N. Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113, 173, 1983.
• 19. MORTELMANS K., ZEIGER E. The Ames Salmonella/microsome mutagenicity assay. Mutat. Res. 455, 29, 2000.
• 20. MONARCA S., FERETTI D., ZERBINI I., ALBERTI A., ZANI C., RESOLA S., GELATTI U., NARDI G. Soil Contamination Detected Using Bacterial and Plant Mutagenicity Tests and Chemical Analyses. Environ. Res. 88, 64, 2002.
• 21. DONNELLY K.C., THOMAS J.C., BROWN K.W. Mutagenic potential of environmental samples before and after remediation of a solvent-contaminated site. Environ. Toxicol. Chem. 14, 281, 1995.
• 22. BIERKENS J., KLEIN G., CORBISER P., VAN DEN HEUEL R., VERSCHAEVE L., WELTENS R., SCHOETERS G. Comparative sensitivity of 20 bioassays for soil quality. Chemosphere 37, 2935, 1998.
• 23. WHITE P., CLAXTON L.D. Mutagens in contaminated soils: a review. Mutat. Res. 567, 227, 2004.
• 24. BARBEE G.C., BROWN K.W., THOMAS J.C., DONELLY K.C., MURRAY H.E. Mutagenic activity (Ames Test) of Wood-Preserving waste Sludge applied to Soil. Bull. Environ. Contam. Toxicol. 57, 54, 1996.