Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test

• Autorzy: Jin Q.Y. , Feng L.L. , Wang Y.B. , Li P. , Yang J.F. , Teng M. , Chai S.J. , Xing G.X. , Zhang G.P.
• Języki publikacji: EN

Abstrakty

EN

A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2022
• Tom: 25
• Numer: 1
• Opis fizyczny: p.27-34,fig.,ref.

Twórcy

autor

Jin Q.Y.

• Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China

autor

Feng L.L.

• Institute of Agricultural Economics and Information, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China

autor

Wang Y.B.

• School of Public Health, Xinxiang Medical University, Xinxiang 453003, PR China

autor

Li P.

• School of Life Sciences and Basic Medicine, Xinxiang University, Xinxiang 453003, PR China

autor

Yang J.F.

• Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China

autor

Teng M.

• Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China

autor

Chai S.J.

• Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China

autor

Xing G.X.

• Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China

autor

Zhang G.P.

• Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China

Identyfikatory

DOI

10.24425/pjvs.2022.140837