Somatic embryogenesis in cell suspension cultures of olive Olea europaea (L.) 'Chetoui'

• Autorzy: Trabelsi E.B. , Naija S. , Elloumi N. , Belfeleh Z. , Msellem M. , Ghezel R. , Bouzid S.
• Języki publikacji: EN

Abstrakty

EN

Somatic embryogenesis of olive Olea europaea (L.) ‘Chetoui’ was studied using cell suspension cultures initiated from mature leaf-derived calli. Calli were developed on half-strength MS medium supplemented with 10 µM NAA and 2.25 µM 2i-P in the dark. Different combinations of three plant growth regulators (2,4-D, NAA and zeatin) were tested to determine cell proliferation and somatic embryogenesis induction and differentiation. Embryogenic suspension cultures were established in olive-modified medium for embryogenesis (OMe) containing 2.5 µM 2,4-D and 2.5 µM zeatin. Pre-globular and globular embryos were induced from mature olive tissue in liquid medium. In addition, the nitrogen form as inorganic (reduced; (NH₄)₂SO₄ or oxidized; KNO₃) and organic (CH) was used separately or in combination to improve the cell growth and proliferation. The most effective growth rate and cell proliferation were obtained with the medium containing inorganic and organic nitrogen forms.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 2
• Opis fizyczny: p.319-324,fig.,ref.

Twórcy

autor

Trabelsi E.B.

• Laboratory of Plant Biology and Biotechnology, Faculty of Sciences of Tunis, Campus Universitaire,1060 Tunis, Tunisia
• Laboratory of Biotechnology and Plant Physiology, The National Institute of Agronomic Research of Tunisia, Rue Hedi Karray, 2049 Ariana, Tunisia
• BP no2, 2040 Rades, Tunisia

autor

Naija S.

• Laboratory of Plant Biology and Biotechnology, Faculty of Sciences of Tunis, Campus Universitaire, 1060 Tunis, Tunisia
• Laboratory of Biotechnology and Plant Physiology, The National Institute of Agronomic Research of Tunisia, Rue Hedi Karray, 2049 Ariana, Tunisia

autor

Elloumi N.

• Laboratory of Biotechnology and Plant Physiology, The National Institute of Agronomic Research of Tunisia, Rue Hedi Karray, 2049 Ariana, Tunisia

autor

Belfeleh Z.

• Laboratory of Biotechnology and Plant Physiology, The National Institute of Agronomic Research of Tunisia, Rue Hedi Karray, 2049 Ariana, Tunisia

autor

Msellem M.

• Institut de l'Olivier de Tunisie, Rue Hedi Karray, 2049 Ariana, Tunisia

autor

Ghezel R.

• Laboratory of Biotechnology and Plant Physiology, The National Institute of Agronomic Research of Tunisia, Rue Hedi Karray, 2049 Ariana, Tunisia

autor

Bouzid S.

• Laboratory of Plant Biology and Biotechnology, Faculty of Sciences of Tunis, Campus Universitaire, 1060 Tunis, Tunisia

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