The aqueous extracts of leaf of Citrus aurantifolia L were assessed in vitro for inhibitory activity against Macrophomina phaseolina isolated from dry root rot specimens of Gingelly. The antifungal activity was determined by poison food technique. The extracts have shown dose dependent inhibition of mycelial growth of test fungi. The extracts were more effective in inhibiting Macrophomina phaseolina. The extracts of Citrus aurantifolia were found effective against Gingelly dry root rot pathogens. Further field experiments are to be carried out to recommend the extracts against the disease.