Usefulness of cytological evaluation of milk in diagnosing mastitis in bitches

• Autorzy: Vasiu I. , Tabaran F. , Pop R.A. , Brudasca F.G. , Tvarijonaviciute A. , Dabrowski R.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to evaluate the prevalence of mastitis in female lactating dogs by interpreting the milk cytology in the diagnostic protocol. Milk samples were manually collected in sterile vials from 89 lactating bitches (i.e., 157 mammary glands) aged between 10 and 168 months. Milk smears were obtained by using the squash and May-Grünwald-Giemsa (MGG) techniques. Acute mastitis episodes were consistent with increased numbers of degenerated neutrophils, erythrocytes, and engulfed bacteria. Eosinophils were present in retention mastitis inflammations. Foamy cells were present in every type of inflammation. Subclinical cases of mastitis were characterized by a reduced number of neutrophils with engulfed bacteria. The period of lactation has a great influence on the type of mastitis encountered. Our results have showed that the lactation period has influence over different types of mammary gland inflammations. In most cases, galactostasis (65%) followed by subclinical mastitis (22%) and acute mastitis (13%) were encountered. Neutrophils and phagocytosis exhibited specificity for acute inflammations, whereas eosinophils and foamy cells were consistent with cases of retention mastitis. The presence of red blood cells in most cases was correlated with acute inflammations. It seems that cytological evaluation of milk could be quite helpful in diagnosing mammary gland inflammations.

Słowa kluczowe

EN

cytological evaluation; usefulness; dog; female; bitch; milk; diagnosis; mastitis; inflammation

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2018
• Tom: 74
• Numer: 10
• Opis fizyczny: p.640-645,fig.,ref.

Twórcy

autor

Vasiu I.

• Department of Infectious Disease, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Romania

autor

Tabaran F.

• Department of Pathology and Forensic Medicine, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Romania

autor

Pop R.A.

• Department of Obstetrics and Gynecology, Medicine, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Romania

autor

Brudasca F.G.

• Department of Infectious Disease, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Romania

autor

Tvarijonaviciute A.

• Interlab-UMU, Regional Campus of International Excellence “Mare Nostrum”, University of Murcia, Murcia, Spain

autor

Dabrowski R.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

Bibliografia

• Biddle D., Macintire D. K.: Obstetrical emergencies. Clin. Tech. Small Anim. Pract. 2000, 15, 88-93.
• Day J. M. (ed.): Clinical Immunology of the Dog and Cat, Manson Publishing/The Veterinary Press, London 2008, p. 11-59.
• Dziecioł M., Stefaniak T., Twardon J., Kozdrowski R.: Wybrane wskazniki mleka i krwi suk ze zdrowym i chorym gruczolem sutkowym (in Polish), [Chosen parameters of the milk and blood of bitches with healthy mammary glands and those suffering from mastitis], Med. Weter. 2006, 62, 59-61.
• Fontaine E., Tanneur M. L., Josein A.: Mammite gangreneuse chez la chienne reproductrice (in French). Le point Vétérinaire 2007, 276, 25-29.
• Jones E. D., Joshua O. J. (eds): Reproductive Clinical Problems in the Dog, Wright PSG, Bristol 1988, p. 113-123.
• Jung C., Wehrend A., König A., Bostedt H.: Investigations about the incidence, differentiation and microbiology of canine mastitis (in German). Prakt. Tierarzt 2002, 83, 508-511.
• Koss L. G., Melamed M. R.: The breast, [in:] Koss L. G., Melamed M. R. (eds): Diagnostic Cytology and its Histopatologic Base. Lippincott Williams & Wilkins, Baltimore 2006, p. 1082-1147.
• Lopate C. (ed.): Management of Pregnant and Neonatal Dogs, Cats and Exotic Pets, Willey-Blackwell, Iowa 2012, p. 25-41.
• Manuali E., Eleni C., Giovaninni P., Costarelli S., Ciorba A.: Unusual finding in a nipple discharge of a female dog: dirofilariasis of the breast. Diagn. Cytopathol. 2004, l32, 108-109.
• Martín R., Olivares M., Pérez M., Xaus J., Torre C., Fernández L., Rodríguez J.: Identification and evaluation of the probiotic potential of lactobacilli isolated from canine milk. Vet. J. 2010, 185, 193-198.
• Meyer J. D., Connolly S. L., Heng H. G.: The acquisition and management of cytology specimens, [in:] Meyer J. D., Connolly S. L., Heng H. G. (eds): Canine and Feline Cytology, a Color Atlas and Interpetation Guide. Saunders Elsevier, St Louis 2010, p. 1-15.
• Olson N. P., Olson A. L.: Cytologic evaluation of canine milk. Vet. Med. Small Anim. Clin. 1984, 79, 641-646.
• Orfanou D. C., Pourlis A., Ververidis H. N., Mavrogianni V. S., Taitzoglou I. A., Boscos C. M., Fthenakis G. C.: Histological features in the mammary glands of female dogs throughout lactation. Anat. Histol. Embryol. 2010, 39, 473-478.
• Reece W. (ed.): Functional Anatomy and Physiology of Domestic Animals. Lippincott Williams & Wilkins, Baltimore 2005.
• Sangha S., Singh A., Sood N. K., Gupta K.: Specificity and sensitivity of cytological techniques for rapid diagnosis of neoplastic and non-neoplastic lesions of canine mammary gland. Braz. J. Vet. Pathol. 2011, 4, 13-22.
• Seweryn T., Boryczko Z.: Bacterial inflammation of mammary gland in the bitch (in Polish). Życie Wet. 2009, 84, 138-144.
• Tizard I.: Immunologic Diseases, [in:] The Merck Veterinary Manual. Whitehouse Station Merck and Co. Inc, New Jersey 2010, p. 741-756.
• Trasch K., Wehrend A., Bostedt H.: Ultrasonographic description of canine mastitis. Vet. Radiol. Ultr. 2007, 48, 580-584.
• Vasiu I., Dąbrowski R., Martinez-Subiela S, Ceron J. J., Wdowiak A., Pop Al. R., Brudaşcă F. Gh.: Milk C-reactive protein in canine mastitis, Vet. Immunol. Immunopathol. 2017, 186, 41-44.
• Vasiu I., Spînu M., Pop Al. R., Bedecean I., Sarpataki O., Brudaşcă F. Gh.: Mastitis acuta in a Hungarian Viszla bitch, caused by a Staphylococcus intermedius infection. Rev. Rom. Med. Vet. 2015, 25, 51-54.
• Ververidis H. N., Mavro Gianni V. S., Fragkou I. A., Orfanou D. C., Gougoulis D. A., Tzivara A., Gouletsou P. G., Athanasiou L., Boscos C. M., Fthenakis G. C.: Experimental Staphylococcal mastits in bitches: Clinical, bacteriological, cytological, haematological, and pathological features. Vet. Microbiol. 2007, 124, 95-106.

Identyfikatory

DOI

10.21521/mw.5963