Paclobutrazol and plant-growth promoting bacterial endophyte Pantoea sp. enhance copper tolerance of guinea grass (Panicum maximum) in hydroponic culture

• Autorzy: Huo W. , Zhuang C. , Cao Y. , Pu M. , Yao H. , Lou L. , Cai Q.
• Języki publikacji: EN

Abstrakty

EN

As most gramineous plants, guinea grass (Panicum maximum) comprise cellulosic biomass, which may be used as a feedstock for bioenergy. In order to develop such potential energy plants on copper-polluted lands, the hydroponic experiments with Cu, Paclobutrazol (PP333, a kind of antigibberellin) and plant growth-promoting bacterial endophyte (PGPB) treatments were carried out in a greenhouse. The seedlings of two cultivars of guinea grass, GG1 (P. maximum var. Natsukomaki) and GG2 (P. maximum var. Natsukaze) in 3 weeks old were treated, respectively, with different Cu treatments [0(CK), 100, 200, 300, 400, 500 µM l⁻¹ Cu] for estimating Cu toxicity. The results showed that elevated Cu restrained plant growth and reduced biomass. According to the EC50 value [the Cu concentration when the relative gain in fresh weight ratio was 50% of control] of two tested cultivars, the concentration of Cu for further experiments was decided as 300 µM l⁻¹. Both pretreatments of PP333 (200, 400, 600 mg l⁻¹) and PGPB (Pantoea sp.) significantly alleviated the negative affect caused by stress of 300 µM l⁻¹ Cu. The pretreatment of 400 mg l⁻¹ PP333 promoted both two cultivars in biomass, compared to 300 µM l⁻¹ Cu treat. The inoculation of Pantoea sp. Jp3-3 increased shoot dry weight, compared to Cu treat. The results suggested that the main reason for both PP333 and Pantoea sp. Jp3-3 enhanced Cu tolerance in guinea grass was that their pretreatments significantly decreased Cu absorption and accumulation under excessive Cu stress. The present study has provided a new insight into the exploitation of energy plant in heavy metal polluted condition by the way of plant growth regulation for increasing heavy metal tolerance.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 1
• Opis fizyczny: p.139-150,fig.,ref.

Twórcy

autor

Huo W.

• College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Zhuang C.

• College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Cao Y.

• College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Pu M.

• College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Yao H.

• College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Lou L.

• College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China

autor

Cai Q.

• Department of Plant Science, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China

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