Inhibition of Digestive α-Amylases from Chilo Suppressalis Walker (Lepidoptera: Crambidae) by a Proteinaceous Extract of Citrullus Colocynthis L. (Cucurbitaceae)

• Autorzy: Valizadeh B. , Zibaee A. , Sendi J. J.
• Języki publikacji: EN

Abstrakty

EN

The striped rice-stem borer, Chilo suppressalis (Walker) is one of the most important pest of rice worldwide. In this study, a proteinaceous extract from Citrullus colocynthis L. shows various degrees of inhibition to digestive α-amylases of C. suppressalis. Digestive α-amylases of larvae were inhibited by different concentrations (approximately 50%) of C. colocynthis amylase inhibitor (CCAI). One of the isozymes totally disappeared and the sharpness of another decreased on native-PAGE electrophoresis. The pH dependency of inhibition revealed that the enzyme was inhibited in a wide range of pH from 7-10, and the optimal pH of the enzyme occurred in lepidopteran larvae. The highest inhibition of α-amylase by CCAI was observed at 25°C; the temperature near the optimal temperature of amylolytic activity. A time-course experiment demonstrated that enzymatic activity was the highest, 30 min after the onset of the experiment, when the highest inhibition occurred. The enzyme kinetic studies using Lineweaver-Burk analysis, revealed a mixed inhibition of CCAI on α-amylase activity. The current study is the first basic one using α-amylase inhibitor against C. suppressalis. This study opens the way for transgenic rice varieties containing inhibitors.

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2013
• Tom: 53
• Numer: 3
• Opis fizyczny: p.195-202,fig.,ref.

Twórcy

autor

Valizadeh B.

• University of Guilan, Department of Plant Protection, Faculty of Agricultural Science, Rash Iran, 41637-1314

autor

Zibaee A.

• University of Guilan, Department of Plant Protection, Faculty of Agricultural Science, Rash Iran, 41637-1314

autor

Sendi J. J.

• University of Guilan, Department of Plant Protection, Faculty of Agricultural Science, Rash Iran, 41637-1314

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