Spinal cord hydrolisate epitopes ameliorate immunological reaction in experimental allergic encephalomyelitis/EAE/

• Autorzy: Kwiatkowska-Patzer B. , Michalkiewicz J. , Zalewska T. , Walski M. , Kurzepa K. , Lipkowski A.W.
• Języki publikacji: EN

Abstrakty

EN

The aim of our study was to evoke oral tolerance with hydrolisate of spinal cord of pig used for feeding the experimental animals/ rats/After induction of tolerance animals were immunised by injection of guine pig spinal cord homogenate with Freund’s adjuvant to induce of EAE, which is an animal model of sclerosis multiplex. Clinical course was have been observed, histopatological study ,ultramicroscopic study and metalloproteinases determination in serum were done. Study of lymphocytes proliferation and level of cytokines IL-4,Il-6, Inf-gamma and TGF-beta were also performed. Clinical course of EAE after hydrolisate treatment has been milder than control. TNF in brains was decreased. Metalloproteases increased in EAE, after hydrolisate treatment were diminished by 30%. Some changes in blood brain barrier/BBB/ as opened tight junction and other changes in early phase of EAE as karioskeletal damage with vesicular structures in karioplasm, compartmentalisation of the endoplasmic reticulum in numbers of phagolisosomes, desorganisation of sheets myelin, neoangiogenesis of parenchyma of the cerebral cortex has been diminished. Mechanism of this effects is probably through active suppression involving diminishing production of IL-4 and interferon gamma as well as increasing production of Il-10 and TGF alfa. All above results indicate that mixture of neuropeptides in spinal cord hydrolisate given orally decrease immunological response to myelin antigens and gave an implication for using oral tolerance to support multiple sclerosis/ MS/ treatment.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: 1
• Opis fizyczny: p.177

Twórcy

autor

Kwiatkowska-Patzer B.

• Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Michalkiewicz J.

• Child Health Centre, Warsaw, Poland

autor

Zalewska T.

• Child Health Centre, Warsaw, Poland

autor

Walski M.

• Child Health Centre, Warsaw, Poland

autor

Kurzepa K.

• Industrial Chemistry Research Institute, Warsaw, Poland

autor

Lipkowski A.W.

• Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
• Industrial Chemistry Research Institute, Warsaw, Poland