Studies on flavanolignans from cultured cells of Silybum marianum

• Autorzy: Elwekeel A. , AbouZid S. , Sokkar N. , Elfishway A.
• Języki publikacji: EN

Abstrakty

EN

Callus and cell suspension cultures of Silybum marianum (L.) Gaertn. (Asteraceae) were established from in vitro germinated sterile plantlets. The cultures grew in Murashige and Skoog medium containing 1 mg l⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg l⁻¹ kinetin. A reversed phase-high performance liquid chromatography method for determination of flavanolignans in plant material was developed using an isocratic solvent system comprising acetonitrile and water containing 0.5% (v/v) phosphoric acid. Silychristin was the major flavanolignan produced by the cultured cells followed by silydianin. Elicitation of cultured S. marianum cells with 100 µg ml⁻¹ yeast extract increased silychristin production from 0.11 to 0.23 mg g⁻¹ fresh weight. Free radical scavenging activity was tested for the cultured cells using 1,1-diphenyl picryl hydrazyl (DPPH) radical. Extract prepared from the cultured cells of S. marianum showed 48% inhibition compared to 55% inhibition of the extract prepared from the fruits. Cytotoxic activity was tested using liver carcinoma cell line (HEPG2). Cultured cells and fruit extracts showed a significant cytotoxic activity of IC₅₀ = 1.01 and 0.47 µg, respectively. Extract of S. marianum cultured cells ameliorated the adverse effects of carbon tetrachloride-induced hepatic injury in rats and returned the altered levels of biochemical markers to near normal levels.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 4
• Opis fizyczny: p.1445-1449,fig.,ref.

Twórcy

autor

Elwekeel A.

• Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, 62111 Beni-Suef, Egypt

autor

AbouZid S.

• Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, 62111 Beni-Suef, Egypt

autor

Sokkar N.

• Pharmacognosy Department, Faculty of Pharmacy, Cairo University, 11562 Cairo, Egypt

autor

Elfishway A.

• Pharmacognosy Department, Faculty of Pharmacy, Cairo University, 11562 Cairo, Egypt

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