Evaluation of the activity of thermostable DNA polymerases in the presence of heme, as a key inhibitor in the real time PCR method in diagnostics of sepsis

• Autorzy: Gosiewski T. , Brzychczy-Wloch M. , Pietrzyk A. , Sroka A. , Bulanda M.
• Języki publikacji: EN

Abstrakty

EN

The study aim was evaluation of the usefulness of several thermostable DNA polymerases in real time PCR conducted in the presence of the heme. Our study had the advantage of testing several different polymerases, one of which proved to be the least sensitive to heme activity. We also found that there is no need of supplementing the reaction mixture with protective substances like BSA. Selection of the appropriate polymerase can increase the efficiency of the PCR reaction which is very important for diagnosis of sepsis and for other analyses performed on DNA template isolated from the blood.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 4
• Opis fizyczny: p.603-606,fig.,ref.

Twórcy

autor

Gosiewski T.

• Chair of Microbiology, Jagiellonian University Medical College, Krakow, Poland

autor

Brzychczy-Wloch M.

• Chair of Microbiology, Jagiellonian University Medical College, Krakow, Poland

autor

Pietrzyk A.

• Chair of Microbiology, Jagiellonian University Medical College, Krakow, Poland

autor

Sroka A.

• Chair of Microbiology, Jagiellonian University Medical College, Krakow, Poland

autor

Bulanda M.

• Chair of Microbiology, Jagiellonian University Medical College, Krakow, Poland

Bibliografia

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