Difference in physiological and biochemical responses to salt stress between Tibetan wild and cultivated barleys

• Autorzy: Jabeen Z. , Hussain N. , Wu D. , Han Y. , Shamsi I. , Wu F. , Zhang G.
• Języki publikacji: EN

Abstrakty

EN

Soil salinity is one of the major abiotic stresses affecting crop growth and yield worldwide. Barley is a species with higher salt tolerance among cereal plants and rich in genetic variation. It is quite important to understand the physiological mechanisms of genotypic difference in salt tolerance. In this study, physiological and biochemical responses of a Tibetan wild barley genotype XZ16 (salt tolerant) and a cultivated cultivar Yerong (salt sensitive) to salt stress were investigated. The results showed that the two genotypes differed dramatically in their responses to salt stress (150 and 300 mM NaCl) in terms of plant biomass, Na+ accumulation and Na+/K+ ratio in roots and shoots, chlorophyll content, xylem sap osmolarity and electrolyte leakage. XZ16 showed less biomass reduction, lower Na+/K+ ratio and electrolyte leakage, higher xylem sap osmolarity, and vacuolar H+-ATPase and H+-PPase activities than Yerong under 300 mM NaCl. The higher salt tolerance of XZ16 may be attributed to its lower concentration of Na+ influx or more sequestration into the vacuoles. The results indicate that the Tibetan wild barley is useful for improvement of cultivated barley in salt stress tolerance.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 09
• Opis fizyczny: Article: 180 [8 p.], fig.,ref.

Twórcy

autor

Jabeen Z.

• Department of Agronomy, Zhejiang University, Hangzhou, 310058, China
• Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, 26000, Pakistan

autor

Hussain N.

• Department of Agronomy, Zhejiang University, Hangzhou, 310058, China

autor

Wu D.

• Department of Agronomy, Zhejiang University, Hangzhou, 310058, China

autor

Han Y.

• Department of Agronomy, Zhejiang University, Hangzhou, 310058, China

autor

Shamsi I.

• Department of Agronomy, Zhejiang University, Hangzhou, 310058, China

autor

Wu F.

• Department of Agronomy, Zhejiang University, Hangzhou, 310058, China

autor

Zhang G.

• Department of Agronomy, Zhejiang University, Hangzhou, 310058, China

Bibliografia

• Ayalew L, Katsuhara M (2009) Insights into the salt tolerance mechanism in barley (Hordeum vulgare) from comparisons of cultivars that differ in salt sensitivity. J Plant Res 123:105–118
• Blumwald E, Aharon GS, Apse MP (2000) Sodium transport in plant cells. Biochim Biophys Acta 1465:140–151
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgam quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Chen Z, Newman I, Zhou M, Mendham N, Zhang G, Shabala S (2005) Screening plants for salt tolerance by measuring K+ flux: a case study for barley. Plant Cell Environ 28:1230–1246
• Chinnusamy V, Jagendore A, Zhu JK (2005) Understanding and improving salt tolerance in plants. Crop Sci 45:437–448
• Dai HX, Shan WN, Zhao J, Zhang GP, Li C, Wu FB (2011) Difference in response to aluminum stress among Tibetan wild barley genotypes. J Plant Nutr Soil Sci 174:952–960
• Dai F, Nevo E, Wu DZ, Comadran J, Zhou MX, Qiu L, Cheng ZH, Belles A, Chen GX, Zhang GP (2012) Tibet is one of the centers of domestication of cultivated barley. Proc Natl Acad Sci USA 109:16969–16973
• Dietz KJ, Tavakoli N, Kluge C, Mimura T, Sharma SS, Harris GC (2001) Significance of the V-type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level. J Exp Bot 52:1969–1980
• Ellis R, Foster B, Handley L, Gordon D, Russell J, Powell W (2000) Wild barley: a source of genes for crop improvement in the 21st century. J Exp Bot 51:9–17
• Fernandez GN, Hernandez M, Casado-Vela J, Bru R, Elortza F, Hedden P, Olmos E (2011) Changes to the proteome and targeted metabolites of xylem sap in Brassica oleracea in response to salt stress. Plant Cell Environ 34:821–836 Flower TJ (2004) Improving crop salt tolerance. J Exp Bot 55:307–319
• Khan NA (2003) NaCl_inhibited chlorophyll synthesis and associated changes in ethylene evolution and antioxidative enzyme activities in wheat. Biol Planta 47:43–74
• Kluge C (2003) New insight into the structure and regulation of the plant vacuolar H+-ATPase. J Bioenerg Biomembr 35:377–388
• Li JY, Jiang GQ, Huang P, Ma J, Zhang FC (2007) Over expression of the Na+/H+ antiporter gene from Suaeda salsa confers cold and salt tolerance to transgenic Arabidopsis thaliana. PCTOC 90:41–48
• Maeshima M (2000) Vacuolar H+ pyrophosphatase. Biochim Biophys Acta 1465:37–51
• Munns R (2005) Genes and salt tolerance: bringing them together. New Phytol 167:645–663
• Porra RJ, Thompson WA, Kriedmann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochem Biophys Acta 975:384–394
• Qiu L, Wu DZ, Shafaqat A, Cai SG, Dai F, Jin XL, Wu FB, Zhang GP (2011) Evaluation of salinity tolerance and analysis of allelic function of HvHKT1 and HvHKT2 in Tibetan wild barley. Theor Appl Genet 122:695–703
• Queirós F, Fontes N, Silva P, Almeida D, Maeshima M, Gerós H, Fidalgo F (2009) Activity of tonoplast proton pumps and Na+/H+ exchange in potato cell cultures is modulated by salt. J Exp Bot 60:1363–1374
• Radoglou K, Cabral R, Repo T, Hasanagas N, Sutinen ML, Waisel Y (2007) Appraisal of root leakage as a method for estimation of root viability. Plant Biosyst 141:443–459
• Razmjoo K, Heydarizadeh P, Sabzalian MR (2008) Effect of salinity and drought stresses on growth parameters and essential oil content of Matricaria chamomile. Int J Agric Biol 10:451–454
• Shabala S, Shabala L, Volkenburgh E (2003) Effect of calcium on root development and root ion fluxes in salinized barley seedlings. Funct Plant Biol 30:507–514
• Shavrukov Y, Gupta NK, Miyazaki J, Baho MN, Chalmers KJ, Tester M, Langridge P, Collins NC (2010) HvNax3—a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum). Funct Integr Genomic 10:277–291
• Steppuhn H, Genuchten MT, Grieve CM (2005) Root-zone salinity selecting a product-yield index and response functions for crop tolerance. Crop Sci 45:209–220
• Steudle E, Smith JAC, LuE` ttge U (1980) Water relations parameters of individual mesophyll cells of the CAM plant KalanchoeÉ daigremontiana. Plant Physiol 66:1155–1163
• Sze H, Schumacher K, Müller ML, Padmanaban S, Taiz L (2002) A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H+-ATPase. Trends Plant Sci 7:157–161
• Tammam AA, Alhamd MFA, Hemeda MM (2008) Study of salt tolerance in wheat Triticum aestivum L. cultivar Banysoif. Aust J Crop Sci 3:115–125
• Tester M, Davenport R (2003) Na+ tolerance and Na+ transport in higher plants. Ann Bot 91:503–527
• Verslues PE, Agarwal M, Katiyar-Agarwal S, Zhu J, Zhu JK (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J 45:523–539
• Walter V, Spelt CD, Vermeer J, Reale L, Koes FR, Quattrocchio F (2009) An H+ P-ATPase on the tonoplast determines vacuolar pH and flower colour. Nat Cell Biol 10:1456–1462
• Wu DZ, Qiu L, Xu LL, Ye LZ, Chen MX, Sun DF, Chen ZH, Zhang HT, Jin XL, Dai F, Zhang GP (2011) Genetic variation of HvCBF genes and their association with salinity tolerance in Tibetan annual wild barley. PLoS One 6:e22938
• Yoshiyuki N, Kasamo K, Shimosato N, Makoto S, Eiji O (1992) Stimulation of the extrusion of protons and H+-ATPase activities with the decline in pyrophosphatase activity of the tonoplast in intact mung bean roots under high-NaCl stress and its relation to external levels of Ca2+ ions. Plant Cell Physiol 33:139–149
• Zhang WH, Yu BJ, Chen Q, Liu YL (2004) Tonoplast H+-ATPase activity in barley roots is regulated by ATP and pyrophosphate contents under NaCl stress. J Plant Physiol Mol Biol 30:45–52
• Zhao J, Sun HY, Dai HX, Zhang GP, Wu FB (2010) Difference in response to drought stress among Tibet wild barley genotypes. Euphytica 172:395–403
• Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71

Identyfikatory

DOI

10.1007/s11738-015-1920-x