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2015 | 37 | 11 |

Tytuł artykułu

Identification and characterization of differentially expressed microRNAs in response to Rhizoctonia solani in maize

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
MicroRNAs (miRNAs) are a set of small, noncoding RNAs that negatively and post-transcriptionally mediate their respective target mRNAs by directing the target mRNA cleavage or translational repression. Plant miRNAs have been involved in developmental processes and adaption to biotic and abiotic stresses in their environment. The banded leaf and sheath blight (BLSB) caused by Rhizoctonia solani is extremely harmful to maize. To investigate the functions of miRNAs under R. solani inoculation, miRNA expression in R. solani infected maize (Zea mays L.) was profiled using deep sequencing. In total, 41 significantly differentially expressed known miRNAs and 39 novel R. solani-responsive miRNAs were identified, of which 9 identified miRNAs were further validated by qRT-PCR, and 2 important miRNAs were analyzed by in situ hybridization. Target genes were also predicted for these R. solani-responsive miRNAs; most of these putative target genes encoded transcription factors and proteins associated with metabolic processes or stress responses. In addition, the mRNA expression levels of several target genes that negatively correlated with the levels of corresponding miRNAs under R. solani inoculation were validated by qRT-PCR. These findings hypothesized that these miRNAs play an important role in R. solani resistance in maize, highlighting novel molecular mechanisms of R. solani resistance in plants.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

37

Numer

11

Opis fizyczny

Article: 250 [17 p.], fig.,ref.

Twórcy

autor
  • Drug Discovery Research Center of Sichuan Medical University, Luzhou, 646000, Sichuan, China
autor
  • Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China
autor
  • Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
  • Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, 400038, China
autor
  • Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China
autor
  • Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China
autor
  • Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China
autor
  • Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China
autor
  • Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-67db626f-73c6-45e4-a4d9-2d46ac09c36b
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