Field experiment was conducted in 2002–2004 in Dusina Plant Breeding Station to investigate the influence of spring plant cutting and application of selected plant growth regulators (PRGs) on seed yield of white clover cvs. Aura and Wota in the first and second years of utilization. The two-factorial experiment was established as a random block design on plots of 15.5 m2 in four repetitions. The following parameters were analysed: morphological features of leaves, seed yield and its structure and seed quality. PRGs – B-Nine 85 SP (daminozide) and Cycocel 460 SL (chlormequat chloride) – were found to be the most effective. Its application in the first and second years of utilization was found to increase seed yield of both cultivars. The observed yield increase was the result of higher inflorescences density per 1 m2 and higher number of pods per head.