Phytochemical evaluation and comparative antibiocide efficacy of Aqueous, Ethanolic and equal mixture of aqueous and ethanolic (1:1) bark extract of Lannea coromandelica L. procured from Eastern region of India

• Autorzy: Das K.
• Języki publikacji: EN

Abstrakty

EN

The present investigation was evaluating the potential antibacterial activity of three different extracts of the bark of Lannea coromandelica Linn. (LC) tree procured from Eastern India. Extraction of bark separation was carried out using aqueous, ethanol and a mixture of aqueous and ethanol. Microbiocides of all the extracts were separately evaluated against several microorganisms viz. Bacillus substilis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonus aeruginosa, Escherichia coli and Serratia marcescens by agar diffusion technique. The Minimum Inhibition Concentration (MIC) of all the extracts was carried out by the serial dilution method. The results of MIC ranged from 12.5 to 150 mg/ml (all the three extracts). The concentration dependent (**P < 0.01) potential antimicrobial activity was resulted and at the dose of 200 mg/ml, combined aqueous and ethanol extract of LC (LCAE + LCEE) gave significant results against gram positive bacteria where the maximum zone of inhibition was recorded against Streptococcus pyogenes (17.0± 0.05**) followed by Straphyloccus aureus (13.6 ±0.05**). Further, the same extract showed the maximum relative percentage inhibition against Straphyloccus aureus (178.64%) followed by Streptococcus pyogenes (143.42%). Such variation may be due to the effects of choice of solvent and the quantity of the extracted amount and also the geographical source of the plant part. These results represent scientific evidence to support the traditional medicinal uses of LC bark extracts and indicate a promising potential used against the treatment of infectious diseases caused by pathogenic bacteria and also provide scientific evidence for their efficacy to prepare the alternate newer medicine for antibiotics.

Słowa kluczowe

EN

India; Lannea coromandelica; bark extract; water extract; ethanol extract; antimicrobial activity; antibacterial agent; antimicrobial agent; agar diffusion test; minimum inhibitory concentration; inhibition; phytochemical investigation

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2014
• Tom: 21
• Opis fizyczny: p.21-31,fig.,ref.

Twórcy

autor

Das K.

• Department of Pharmacognosy and Phytochemistry, Krupanidhi College of Pharmacy, 12/1, Carmelaram Post, Varthur Hobli. Bangalore-35, India

Bibliografia

• [1] Dhiman A., Lal R., 2011. Phytochemical and pharmacological status of Datura fastuosa Linn. Int J Res Ayurveda Pharm 2(1), 145-150.
• [2] Varahalarao V., Chandrashekar NK., 2010. In Vitro bioactivity of Indian medicinal plant Calotropis procera (Ait.). Journal of Global Pharma Technology 2(2), 43-45.
• [3] Berkowitz FE., 1995. Antibiotic resistance in bacteria. South Med. J 88, 797-804.
• [4] Kumar G., Karthik L., Bhaskara Rao KV., 2010. Antibacterial activity of aqueous extract of Calotropis gigantea leaves – an in vitro study. International Journal of Pharmaceutical Sciences Review and Research 4(2), 141-144.
• [5] Raghunath D., 2008. Emerging antibiotic resistance in bacteria with special reference to India. J Biosci 33 (2008) 593-603.
• [6] Hadia G., Rubina N., Qaisrani MAK., Shazia H., Nabila Y., 2012. Antibacterial and antifungal activity of different extracts of Datura stramonium (branches and leaves sample). J Biotech Pharm Res 3(9), 141-148.
• [7] Irudayaraj V., Janaky M., Johnson M., Selvan N., 2012. Preliminary phytochemical and antimicrobial studies on a spike-moss Selaginella inaequalifolia (hook. & grev.) Spring. Asian Pac J Trop Med 3(12), 957-960. [8] Jain SK., Tarafder CR., 1970. Medicinal plants-lore of the sandals-A revival of PO Bodding's work. Econ Bot 24, 241-278.
• [9] Islam MT, Tahara S., 2000. Dihydroflavonols from Lannea coromandelica. Phytochemistry 54, 901-907.
• [10] Gan BC., Li RT., Yang XQ., Du DL., 2007. Ethnobotany studies on medicinal plants used by Li nationality in Wuzhishan area of Hainan province. Chin J Ethnomed Ethnopharm 4, 194-198.
• [11] Islam MT., Sakasai M., Tahara S., 2002. Zoosporicidal activity of polyflavonoid tannin identified in Lannea coromaldelica stem bark against phytopathogenic oomycete aphanomyces cochlioides. J Agric Food Chem 6(23), 6697-6703.
• [12] Singh S., Singh GB., 2005. Antiinflammatory activity of Lannea coromaldelica bark extract in rat. Phytother Res 8, 311-318.
• [13] Rahman MS., Begum B., Chowdhury R., Rahman KM., Rashid MA., 2008. Preliminary cytotoxicity screening of some medicinal plants of Bangladesh. Dhaka Univ J Pharm Sci 7(1), 47-52.
• [14] Badrul A., Sarowar H., Razibul H., Julia R., Anwarul I., 2012. Antioxidant and analgesic activities of Lannea coromandelica Linn bark extract. International J of Pharmacol 8(4), 224.
• [15] Kaur R., Jaiswal ML., Jain V., 2013. Protective effect of Lannea coromandelica Houtt. Merrill. Against three common pathogens. J Ayurveda Integr Med 4, 224-228.
• [16] Srinivasa Rao V., Einstein JW., Das K., 2014. Hepatoprotective and antioxidant activity of Lannea coromandelica Linn. on thioacetamide induced hepatotoxicity in rats. International Letters of Natural Sciences 3, 30-43.
• [17] Krishnaiah D., Devi T., Bono A., Sarbatly R., 2009. Studies on phytochemical constituents of six Malaysian medicinal plants. Journal of Medicinal Plants Research 3(2), 67-72.
• [18] Rios JL., Recio MC., Vilar A., 1988. Screening methods for natural products with antimicrobial activity: A review of literature. Journal of Ethnopharmacology 23, 127-149.
• [19] Bauer AW., Kirby WM., Sheriss JC., Turck M., 1966 Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45, 493-496.
• [20] Ajay KK., Lokanatha RMK., Umesha KB., 2003. Evaluation of antibacterial activity of 3,5-dicyano-4,6-diaryl-4-ethoxycarbonyl-piperid-2-ones. Journal of Pharmaceutical and Biomedical Analysis 27, 837-840.
• [21] Nkere CK., Lroegbu CU., 2005. Antimicrobial screening of the root, seed and stem bark extracts of Picralima nitida. Afr J Biotechnol 4, 522- 526.
• [22] Das K., Dang R., Gupta N., 2009. Comparative antimicrobial potential of different extracts of leaves of Stevia rebaudiana Bert. Int J Nat Eng Sci 3 (1), 59-62.
• [23] Masotti VF., Juteau JM., Bessiere Viano J., 2003. Seasonal and phonological variations of the essential oil from the narrow endemic species Artemisia molinieri and its biological activities. Journal of Agricultural and Food Chemistry 51, 7115-7121.
• [24] Angioni A., Barra A., Coroneo V., Dessi S., Cabras P., 2006. Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. Stoechas essential oils from stem/ leaves and flowers. Journal of Agricultural and Food Chemistry 54, 4364-4370.
• [25] Bedi N., Bedi PMS., Bodiwala HS., Singh IP., Bansal P., 2010. Scientific evaluation of an innovative herbal medicine for relief in respiratory disorders. Canadian journal of pure & applied sciences 4(3), 1249-1255.
• [26] Bakht J., Tayyab M., Ali H., Islam A., Shafi M., 2011. Effect of different solvent extracted sample of Allium sativum (Linn) on bacteria and fungi. African Journal of Biotechnology 10(31), 5910-5915.
• [27] Basuri TS., Patil C., Dhal NK., 2011. In vitro evaluation of antimicrobial activity of crude ethanolic extract of Lannea coromandelica (Linn.). Journal of Pharmacy Research 4(4), 1246-1247.
• [28] Shimada T., 2006. Salivary proteins as a defense against dietary tannins. J. Chem. Ecol 32(6), 1149-1163.
• [29] Hodek P., Trefil P., Stiborova M., 2002. Flavonoids - Potent and versatile biologically active compounds interacting with cytochrome P450. Chemico-Biol. Intern 139(1), 1-21.
• [30] Islam MT., Tahara S., 2000. Dihydroflavonols from Lannea coromandelica. Phytochemistry 54, 901-907.
• [31] Krishnaiah PV., Ratnam KV., Venkata RR., 2008. Preliminary Phytochemical evaluation of certain anticancer crude drugs used by adivasis of rayalaseema region, Andhra Pradesh, India. Ethn Bot Leaflets 12, 693-697.
• [32] Yun XJ., Shu HM., Chen GV., Ji MH., Ding JY., 2014. Chemical constituents from bark of Lannea coromandelica. Chinese Herbal Medicines 6(1), 65-69.