Use of amplification fragment length polymorphism to genotype Pseudomonas stutzeri strains following exposure to ultraviolet light A

• Autorzy: Lombardi L. , Zoppo M. , Rizzato C. , Egan C.G. , Scarpato R. , Tavanti A.
• Języki publikacji: EN

Abstrakty

EN

Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium Pseudomonas stutzeri, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate the effect of UVA-exposure on genomic DNA extracted from 18 survival colonies of the two strains compared to unexposed controls. AFLP showed a high discriminatory power, confirming the existence of different genotypes within the species and presence of DNA polymorphismsin UVA-exposed colonies.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2017
• Tom: 66
• Numer: 1
• Opis fizyczny: p.107-111,fig.,ref.

Twórcy

autor

Lombardi L.

• Department of Biology, Genetic Unit, University of Pisa, Pisa, Italy

autor

Zoppo M.

• Department of Biology, Genetic Unit, University of Pisa, Pisa, Italy

autor

Rizzato C.

• Department of Biology, Genetic Unit, University of Pisa, Pisa, Italy

autor

Egan C.G.

• Primula Multimedia S.r.L., Pisa, Italy

autor

Scarpato R.

• Department of Biology, Genetic Unit, University of Pisa, Pisa, Italy

autor

Tavanti A.

• Department of Biology, Genetic Unit, University of Pisa, Pisa, Italy

Bibliografia

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Identyfikatory

DOI

10.5604/17331331.1234998