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2015 | 64 | 1 |

Tytuł artykułu

Optimized protocol for PFGE analysis of anginosus (milleri) Streptococci

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Streptococcus anginosus (milleri) is a diverse group of gram positive bacteria. Molecular methods to establish relationship between strains are poorly developed. Therefore, main tool to study genetic variability is restriction fragment length polymorphism combined with pulsed field gel electrophoresis (RFLP-PFGE). In this communication, we present optimized protocol for S. anginosus PFGE analysis.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

64

Numer

1

Opis fizyczny

p.61-64,fig.,ref.

Twórcy

autor
  • Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
  • Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
  • Department of Molecular Microbiology, National Medicines Institute, Warsaw, Poland

Bibliografia

  • Bartie K.L., M.J. Wilson, D.W. Williams and M.A. Lewis. 2000. Macrorestriction fingerprinting of “Streptococcus milleri” group bacteria by pulsed-field gel electrophoresis. J. Clin. Microbiol. 38: 2141–2149.
  • Bens C.C., A. Voss and C.H. Klaassen. 2006. Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis. J. Clin. Microbiol. 44: 1875–1876.
  • Borek A.L., J. Wilemska, R. Izdebski, W. Hryniewicz and I. Sitkiewicz. 2011. A new rapid and cost-effective method for detection of phages, ICEs and virulence factors encoded by Streptococcus pyogenes. Pol. J. Microbiol. 60: 187–201.
  • Borek A.L., K. Obszanska, W. Hryniewicz and I. Sitkiewicz. 2012a. Detection of Streptococcus pyogenes virulence factors by multiplex PCR. Virulence 3: 529–533.
  • Borek A.L., K. Obszanska, W. Hryniewicz and I. Sitkiewicz. 2012b. Typing of Streptococcus pyogenes strains using the phage profiling method. Virulence 3: 534–538.
  • Chang Y.C. and H.H. Lo. 2013. Identification, clinical aspects, susceptibility pattern, and molecular epidemiology of beta-haemolytic group G Streptococcus anginosus group isolates from central Taiwan. Diagn. Microbiol. Infect. Dis. 76: 262–265.
  • Chung M., H. de Lencastre, P. Matthews, A. Tomasz, I. Adamsson, M. Aires de Sousa, T. Camou, C. Cocuzza, A. Corso, I. Couto and others. 2000. Molecular typing of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis: comparison of results obtained in a multilaboratory effort using identical protocols and MRSA strains. Microb. Drug. Resist. 6: 189–198.
  • Facklam R. 2002. What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin. Microbiol. Rev. 15: 613–630.
  • Kohler W. 2007. The present state of species within the genera Streptococcus and Enterococcus. Int. J. Med. Microbiol. 297: 133–150.
  • Lancefield R.C. 1933. A serological differentiation of human and other groups of hemolytic streptococci. J. Exp. Med. 57: 571–595.
  • Obszanska K., A.L. Borek, R. Izdebski, W. Hryniewicz and I. Sitkiewicz. 2011. Multilocus variable number tandem repeat analysis (MLVA) of Streptococcus pyogenes. J. Microbiol. Methods. 87: 143–149.
  • Obszanska K., A.L. Borek, W. Hryniewicz and I. Sitkiewicz. 2012. Multiple locus VNTR fingerprinting (MLVF) of Streptococcus pyogenes. Virulence 3: 539–542.
  • Tenover F.C., R.D. Arbeit, R.V. Goering, P.A. Mickelsen, B.E. Murray, D.H. Persing and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33: 2233–2239.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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