Loss-of-function mutation of EIN2 in Arabidopsis exaggerates oxidative stress induced by salinity

• Autorzy: Lin Y , Chen D. , Paul M. , Zu Y. , Tang Z.
• Języki publikacji: EN

Abstrakty

EN

Accumulation of reactive oxygen species (ROS) causes oxidative stress under adverse environmental conditions, such as salinity. Ethylene decreases accumulation of ROS induced by salinity, but the mechanism is still unclear. To examine the interactions between salinity and ROS accumulation and the possible role of ethylene metabolism in regulation, we used mutant ein2-5 in Arabidopsis with loss of function in EIN2. The mutant is compared to the wild-type Col-0, completely insensitivity to ethylene at the morphological, physiological and molecular levels. The oxidative responses of the wild type and mutant to salinity were compared. Loss-of-function of EIN2 enhanced sensitivity to salinity, implying that EIN2 is required for plant response to salinity. Furthermore, salinity resulted in accumulation of large amounts of ROS in ein2-5 seedlings when compared with Col-0, suggesting that the loss-of-function of EIN2 exaggerates oxidative stress induced by salinity. Activities of the antioxidant enzymes SOD, POD and CAT decreased significantly in ein2-5 under salinity when compared with Col-0 plants. The expression profiles of the genes Fe-SOD, PODs and CAT1, which code for ROS scavenging enzymes were severely decreased in ein2-5 under salinity compared with Col-0, suggesting that EIN2 was involved in regulating expression of these genes. Taken together, our results demonstrate that loss-of-function of EIN2 increased oxidative stress induced by salinity and that EIN2 is involved in modulating ROS accumulation, at least in part, by decreasing activities of ROS-scavenging enzymes.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 04
• Opis fizyczny: p.1319-1328,fig.,ref.

Twórcy

autor

Lin Y

• Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, People’s Republic of China

autor

Chen D.

• Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, People’s Republic of China

autor

Paul M.

• Plant Science, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK

autor

Zu Y.

• Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, People’s Republic of China

autor

Tang Z.

• Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, People’s Republic of China

Bibliografia

• Achard P, Cheng H, Grauwe LD, Decat J, Schoutteten H, Moritz T, Straeten DVD, Peng J, Harberd NP (2006) Integration of plant responses to environmentally activated phytohormonal signals. Science 311:91–94
• Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126
• Alonso JM, Stepanova AN (2004) The ethylene signaling pathway. Science 306:1513–1515
• Alonso JM, Hirayama T, Roman G, Nourizadeh S, Ecker JR (1999) EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science 284:2148–2152
• Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53:1331–1341
• Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399
• Beauchamp C, Fridovic I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
• Bleecker AB, Kende H (2000) Ethylene: a gaseous signal molecule in plants. Annu Rev Cell Dev Biol 16:1–18
• Bouchez O, Huard C, Lorrain S, Robby D, Balague´ C (2007) Ethylene is one of the key elements for cell death and defense response control in the Arabidopsis lesion mimic mutant vad1. Plant Physiol 145:465–477
• Cao WH, Liu J, He XJ, Mu RL, Zhou HL, Chen SY, Zhang JS (2007) Modulation of ethylene responses affects plant salt-stress responses. Plant Physiol 143:707–719
• Cao YR, Chen SY, Zhang JS (2008) Ethylene signaling regulates salt stress response. Plant Signal Behav 3:761–763
• Cao SQ, Chen ZY, Liu GQ, Jiang L, Yuan HB, Ren G, Bian XH, Jian HY, Ma XL (2009) The Arabidopsis ethylene-insensitive 2 gene is required for lead resistance. Plant Physiol Biochem 47:308–312
• Chao Q, Rothenberg M, Solano R, Roman G, Terzaghi W, Ecker JR (1997) Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell 89:1133–1144
• Chen Y, Etheridge N, Schaller GE (2005) Ethylene signaling transduction. Ann Bot 95:901–915
• de Jong AJ, Yakimova ET, Kapchina VM, Woltering EJ (2002) A critical role for ethylene in hydrogen peroxide release during programmed cell death in tomato suspension cells. Planta 214:537–545
• Dong CH, Jang M, Scharein B, Malach A, Rivarola M, Liesch J, Groth G, Hwang I, Chang C (2010) Molecular association of the Arabidopsis ETR1 ethylene receptor and a regulator of ethylene signaling, RTE1. J Biochem 285:40706–40713
• Dunand C, Cre`vecoeur M, Penel C (2007) Distribution of superoxide and hydrogen peroxide in Arabidopsis root and their influence on root development: possible interaction with peroxidases. New Phytol 174:332–341
• Ellouzi H, Hamed KB, Cela J, Munne´-Bosch S, Abdelly C (2011) Early effects of salt stress on the physiological and oxidative status of Cakile maritima (halophyte) and Arabidopsis thaliana (glycophyte). Physiol Plant 142(2):128–143
• Gadjev I, Vanderauwera S, Gechev TS, Laloi C, Minkov IN, Shulaev V, Apel K, Inze´ D, Mittler R, Van Breusegem F (2006) Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis. Plant Physiol 141:436–445
• Gechev TS, Van Breusegem F, Stone JM, Denev I, Laloi C (2006) Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. BioEssays 28:1091–1101
• Grbic´ V, Bleecker AB (1995) Ethylene regulates the timing of leaf senescence in Arabidopsis. Plant J 8:595–602
• Hiscox JD, Israelstam GF (1979) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334
• Jiang M, Zhang J (2001) Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol 42:1265–1273
• Johnson PR, Ecker JR (1998) The ethylene gas signal transduction pathway: a molecular perspective. Annu Rev Genet 32:227–254
• Jung JY, Shin R, Schachtman DP (2009) Ethylene mediates response and tolerance to potassium deprivation in Arabidopsis. Plant Cell 21:607–621
• La¨uchli A, Grattan SR (2007) Plant growth and development under salinity stress. In: Jenks MA, Hasegawa P, Jain SM (eds) Advances in molecular breeding toward drought and salt tolerant crops. Springer, Netherlands, pp 1–32
• Lei G, Shen M, Li ZG, Zhang B, Duan KX, Wang N, Cao YR, Zhang WK, Ma B, Ling HQ, Chen SY, Zhang JS (2011) EIN2 regulates salt stress response and interacts with a MA3 domain-containing protein ECIP1 in Arabidopsis. Plant Cell Environ 34:1678–1692
• Lin Y, Wang J, Zu Y, Tang Z (2012) Ethylene antagonizes the inhibition of germination in Arabidopsis induced by salinity by modulating the concentration of hydrogen peroxide. Acta Physiol Planta 34:1895–1904
• McKersie BD, Murnaghan J, Jones KS, Bowley SR (2000) Ironsuperoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance. Plant Physiol 122:1427–1438
• Miller G, Suzuki N, Ciftci-Yilma S, Mittler R (2010) Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ 33:453–467
• Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9: 490–498
• Pierik P, Tholen D, Poorter H, Visser E, Voesenek L (2006) The Janus face of ethylene: growth inhibition and stimulation. Trends Plant Sci 11:176–183
• Rathmell WG, Sequeira L (1974) Soluble peroxidase in fluid from the intercellular spaces of tobacco leaves. Plant Physiol 53:317–318
• Schopfer P, Plachy C, Frahry G (2001) Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiol 125:1591–1602
• Shin R, Kim K, Kang H, Zulfuarov IS, Bae G, Lee CH, Lee D, Choi G (2009) Phytochromes promote seedling light responses by inhibiting four negative-acting phytochrome-interacting factors. Proc Natl Acad Sci USA 106:7660–7665
• Sun ZH, Chen W, Chen ZY, Zhang QA, Fang L, Li J, Jiang ST, Cao SQ (2011) A role for Ethylene-Insensitive 2 gene in the regulation of the ultraviolet-B response in Arabidopsis. Acta Physiol Planta 33:1025–1030
• Torres MA, Dangl JL (2005) Functions of the respiratory burst oxidase in biotic interactions, abiotic stress and development. Curr Opin Plant Biol 8:397–403
• Van Breusegem F, Dat JF (2006) Reactive oxygen species in plant cell death. Plant Physiol 141:384–390
• Vandenbussche F, Vaseva I, Vissenberg K, Van Der Straeten D (2012) Ethylene in vegetative development: a tale with a riddle. New Phytol 194:895–909
• Wang YN, Liu C, Li K, Sun F, Hu H, Li X, Zhao Y, Han C, Zhang W, Duan Y, Liu M, Li X (2007) Arabidopsis EIN2 modulates stress response through abscisic acid response pathway. Plant Mol Biol 64:633–644
• Wu G, Wilen RW, Robertson AJ, Gusta LV (1999) Isolation, chromosomal localization, and differential expression of mitochondrial manganese superoxide dismutase and chloroplastic copper/zinc superoxide dismutase genes in wheat. Plant Physiol 120:513–520
• Wu LJ, Zhang Z, Zhang H, Wang XC, Huang R (2008) Transcriptional modulation of ethylene response factor protein JERF3 in the oxidative stress response enhances tolerance of tobacco seedlings to salt, drought, and freezing. Plant Physiol 148: 1953–1963
• Yang L, Zu YG, Tang ZH (2010) Ethylene improves Arabidopsis salt tolerance mainly via retaining K? in shoots and roots rather than decreasing tissue Na? content. Environ Exp Bot. doi:10.1016/j.envexpbot.2010.08.006
• Ye N, Zhu G, Liu Y, Zhang A, Li Y, Liu R, Shi L, Jia L, Zhang J (2012) Ascorbic acid and reactive oxygen species are involved in the inhibition of seed germination by abscisic acid in rice seeds. J Exp Bot 63:1809–1822
• Zhang X, Zhang L, Dong F, Gao J, Galbraith DW, Song CP (2001) Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiol 126:1438–1448
• Zhong S, Zhao M, Shi T, Shi H, An F, Zhao Q, Guo H (2009) EIN3EIL1 cooperate with PIF1 to prevent photo-oxidation and to promote greening of Arabidopsis seedlings. Proc Natl Acad Sci USA 106:21431–21436

Uwagi

rekord w opracowaniu