Early postnatal development of the lumbar vertebrae in male Wistar rats: double staining and digital radiological studies

• Autorzy: Burdon F. , Blaszczak-Szalak M. , Rozylo-Kalinowska I. , Klepacz R. , Dworzanski W. , Rozylo T.K. , Dudka J. , Szumilo J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to evaluate the physiological developmental changes of male rats’ lumbar vertebrae during the first 22 days after birth. Morphology and mineralisation of lumbar vertebrae were evaluated using double-staining and digital radiography system, which allowed vertebral width and optical density to be determined. Pup weight, crown-rump length, body mass index and vertebral width increased during postnatal period and significantly correlated with their age. Bone mineralisation, as measured by optical density, did not show any significant differences. The complete fusion of the primary ossification centres had a cranio-caudal direction and started on day 19 after parturition but was incomplete by day 22. It could be concluded that, unlike significant age-related increase of vertebral size, mineralisation was only slightly elevated during evaluated postnatal period. The method described is supplementary to alizarin red S staining as it provides both qualitative and quantitative data on mineralisation in a similar manner to micro computed tomography but does not allow 3 dimensional and microarchitecture examination. (Folia Morphol 2016; 75, 1: 1–13)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2016
• Tom: 75
• Numer: 1
• Opis fizyczny: p.1-13,fig.,ref.

Twórcy

autor

Burdon F.

• Experimental Teratology Unit, Human Anatomy Department, Medical University of Lublin, Jaczewskiego 4, 20–090 Lublin, Poland
• Radiology Department, St. John’s Cancer Centre, Lublin, Poland

autor

Blaszczak-Szalak M.

• Human Anatomy Department, Medical University of Lublin, Lublin, Poland

autor

Rozylo-Kalinowska I.

• Independent Unit of Propaedeutic of Dental and Maxillofacial Radiology, Medical University of Lublin, Lublin, Poland

autor

Klepacz R.

• Clinical Pathomorphology Department, Medical University of Lublin, Lublin, Poland

autor

Dworzanski W.

• Human Anatomy Department, Medical University of Lublin, Lublin, Poland

autor

Rozylo T.K.

• Dental and Maxillofacial Radiology Department, Medical University of Lublin, Lublin, Poland

autor

Dudka J.

• Clinical Pathomorphology Department, Medical University of Lublin, Lublin, Poland

autor

Szumilo J.

• Clinical Pathomorphology Department, Medical University of Lublin, Lublin, Poland

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Identyfikatory

DOI

10.5603/FM.a2015.0068