Development of mutants of Coniothyrium minitans with improved efficiency for control of Sclerotinia sclerotiorum

• Autorzy: Melo I.S. , Moretini A. , Cossiolato A. M. R. , Faull J. L.

Warianty tytułu

PL

Mutanty grzyba Coniothyrium minitans o zwiększonej skuteczności w zwalczaniu patogena Slerotinia sclerotiorum

• Języki publikacji: EN

Abstrakty

EN

Coniothyrium minitans (CM) is hyperparasitic to Sclerotinia sclerotiorum (SS), a pathogen of many economically important crops. In this paper, we describe the isolation of improved mutants of CM, using a UV - irradiation regime, with altered chitinase production and tolerance to high concentration of iprodione, which are effective against SS. Three out of the 59 mutants obtained inhibited the mycelial growth of CM. Infectivity of sclerotia by the new mutants was assayed by the plant-tissue-based system using carrot segments. More than 80% of sclerotia were colonized by the mutants and the wild-type CM. The mutant strains retained ability to produce significant amounts of chitinase. The mutants differed from their wild-type strain in appearance, morphology and sporulation. In conclusion, the results presented here provide evidence that the new biotypes of C. minitans are effective in controlling S. sclerotiorum.

PL

Grzyb Coniothyrium minitans jest nadpasożytem innego gatunku grzyba, Sclerotinia sclerotiorum - patogena wielu ważnych gospodarczo roślin uprawnych. W pracy przedstawiono metodę wykorzystania promieni ultrafioletowych (UV) w celu uzyskania mutantów grzyba C. minitans o zmodyfikowanym wytwarzaniu enzymu chitynazy, tolerancji na wysokie dawki fungicydu iprodion oraz skutecznych przeciwko patogenowi S. sclerotiorum. Spośród 59 zmutowanych izolatów, trzy izolaty inhibowały wzrost grzybni testowanego patogena. Zainfekowanie sklerot grzyba S. sclerotiorum przez nowe zmutowane izolaty oceniano na żywym materiale roślinnym (plasterki marchwi). Ponad 80% sklerot zostało zainfekowanych przez zmutowane izolaty oraz izolaty C. minitans występujące w naturalnym środowisku. Zmutowane izolaty zachowywały trwałą zdolność do produkcji większych ilości chitynazy oraz wykazywały różnice dotyczące morfologii i zarodnikowania grzyba. Biorąc pod uwagę wyniki prezentowanych badań można wnioskować, że nowe biotypy grzyba C. minitans są skuteczne w zwalczaniu patogena S. sclerotiorum.

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2011
• Tom: 51
• Numer: 2
• Opis fizyczny: p.179-183,fig.,ref.

Twórcy

autor

Melo I.S.

• Brazilian Agricultural Research Corporation-Embrapa Environment, C.P.69, 13820-000, Jaguariuna-SP, Brazil

autor

Moretini A.

autor

Cossiolato A. M. R.

autor

Faull J. L.

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