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2014 | 70 | 11 |

Tytuł artykułu

Detection of Salmonella spp., Salmonella enteritidis, Salmonella typhi and Salmonella typhimurium in cream cakes by polymerase chain reaction (PCR)

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aims of this study were: (i) to determine the incidence of Salmonella spp. by conventional culture methods in cream cakes, (ii) to detect invA gene by PCR for the confirmation of the isolates, (iii) to analyze isolates for S. Enteritidis, S. Typhi, and S. Typhimurium by PCR based on Sdf I, ViaB, Spy gene sequences, respectively, and (iv) to identify isolates in comparison to the reference strain by DNA sequence analysis. A total of 81 unpackaged cream cake samples were obtained from different patisseries in Hatay, Turkey. Salmonella spp. was detected in 13 (16%) out of 81 cream cake samples by the conventional culture method. Atotal of 45 isolates from the 13 positive samples were confirmed as Salmonella spp. by PCR. Homology among the reference strain and isolates and homology within the isolates was found to be 98.97-100%. Cream cake samples analyzed in this study were found to be contaminated with Salmonella, thus posing a potential health hazard for the consumer. To protect public health, food safety management systems such as HACCP, GMP and GHP could be carried out in cream cake production.

Wydawca

-

Rocznik

Tom

70

Numer

11

Opis fizyczny

p.689-692,fig.,ref.

Twórcy

autor
  • Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31034 Hatay, Turkey
autor
  • Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31034 Hatay, Turkey
autor
  • Microbiology Reference Laboratories Department, Public Health Institutes of Turkey, 06100 Ankara, Turkey
autor
  • Department of Hotel, Restaurant and Catering Services, Milas Vocational School, Mugla Sitki Kocman University, 48200, Turkey

Bibliografia

  • 1. Agron P. G., Walker R. L., Kinde H., Sawyer S. J., Hayes D. C., Wollard J., Andersen G. L.: Identification by subtractive hybridization of sequences specific for Salmonella enterica serotype Enteritidis. Appl. Environ. Microbiol. 2001, 67, 4984-4991.
  • 2. Akiba M., Kusumoto M., Iwata T.: Rapid identification of Salmonella enterica serovars, Typhimurium, Choleraesuis, Infantis, Hadar, Enteritidis, Dublin and Gallinarum, by Multiplex PCR. J. Microbiol. Methods 2011, 85, 9-15.
  • 3. D’Aoust J. Y, Maurer J.: Salmonella species, [in:] Doyle M. P., Beuchat L. R. (ed.), Food Microbiology: Fundamentals and Frontiers, Washington, USA, ASM Press 2007, 187-236.
  • 4. Elmali M., Ulukanli Z., Yaman H., Genctav K.: Hygienic quality of cream pastries in Turkey. Arch. Lebensmittelhyg. 2007, 58, 14-18.
  • 5. Freitas C. G. de, Santana A. P., Da Silva P. H. C., Goncalves V. S. P., Barros M. A. F., Torres F. A. G., Murata L. S., Perecmanis S.: PCR multiplex for detection of Salmonella Enteritidis, Typhi and Typhimurium and occurence in poultry meat. Int. J. Food Microbiol. 2010, 139, 15-22.
  • 6. Gumus T., Daglıoglu O., Konyalı A. M.: Microbiological quality of cream cakes sold in Tekirdag province. J. Tekirdag Agric. Fac. 2005, 2, 215-220.
  • 7. Herikstad H., Motarjemi Y., Tauxe R. V.: Salmonella surveillance: a global survey of public health serotyping. Epidemiol. Infect. 2002, 129, 1-8.
  • 8. Jay J. M., Loessner M. J., Golden D. A.: Foodborne gastroenteritis caused by Salmonella and Shigella. Springer 2005, Chapter 26, 619-630.
  • 9. Kotzekidou P.: Microbiological examination of ready-to-eat foods and readyto-bake frozen pastries from university canteens. Food Microbiol. 2013, 34, 337-343.
  • 10. Kumar S., Balakrishna K., Batra H. V.: Detection of Salmonella enterica serovar Typhi (S. Typhi) by selective amplification of invA, viaB, fliC-d and prt genes by polymerase chain reaction in multiplex format. Lett. Appl. Microbiol. 2006, 42, 149-154.
  • 11. Malorny B., Hoorfar J., Hugas M., Heuvelink A., Fach P., Ellerbroek L., Bunge C., Dorn C., Helmuth R.: Interlaboratory diagnostic accuracy of a Salmonella specific PCR-based method. Int. J. Food Microbiol. 2003, 89, 241-249.
  • 12. Meldrum R. J., Smith R. M. M., Ellis P., Garside J.: Microbiological quality of randomly selected ready-to-eat foods sampled between 2003 and 2005 in Wales, UK. Int. J. Food Microbiol. 2006, 108, 397-400.
  • 13. Olsen J. E., Aabo S., Rasmussen O. F., Rossen L.: Oligonucleotide probes specific for the genus Salmonella and for Salm. typhimurium. Lett. Appl. Microbiol. 1995, 20, 160-163.
  • 14. Öktem A. B., Onurdağ F. K., Er B., Demirhan B.: A research of Salmonella spp. in egg and egg products and survival of Salmonella in different temperatures. Turkish J. Pharm. Sci. 2009, 6, 147-154.
  • 15. Piknova L., Stefanovicova A., Drahovska H., Sasik M., Kuchta T.: Detection Salmonella in food, equivalent to ISO 6579, by a three-days polymerase chain reaction- based method. Food Cont. 2002, 13, 191-194.
  • 16. Pui C. F., Wong W. C., Chai L. C., Lee H. Y., Noorlis A., Zainazor T. C. T., Tang J. Y. H., Ghazali F. M., Cheah Y. K., Nakaguchi Y., Nishibuchi M., Radu S.: Multiplex PCR for the concurrent detection and differentiation of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium. Trop. Med. Health 2011, 39, 9-15.
  • 17. Rahn K., De Grandis S. A., Clarke R. C., McEwen S. A., Galan J. E., Ginocchio C., Curtiss R. 3rd, Gyles C. L.: Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol. Cell. Probe 1992, 6, 271-279.
  • 18. Siriken B., Cadirci O., Inat G., Pamuk S.: Microbiological examination of meatball, cream cake and turkish delight (lokum). J. Anim. Vet. Adv. 2009, 8, 2049-2054.
  • 19. Smith J. P., Daifas D. P., El-Khoury W., Koukoutsis J., El-Khoury A.: Shelf life and safety concerns of bakery products-A review. Critic. Rev. Food Sci. Nutr. 2004, 44, 19-55.
  • 20. Solhan S., Chan P. P., Kurupatham L., Foong B. H., Ooi P. L., James L., Phua L., Tan A. L., Koh D., Goh K. T.: An outbreak of gastroenteritis caused by Salmonella enterica serotype Enteritidis traced to cream cakes. WPSAR 2011, 2, 23-30.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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