Nitric oxide alleviates manganese toxicity by preventing oxidative stress in exised rice leaves

• Autorzy: Srivastava S. , Dubey R. S.
• Języki publikacji: EN

Abstrakty

EN

In the present study, we have investigated the effects of nitric oxide (NO) on alleviating manganese (Mn)-induced oxidative stress in rice leaves. Exogenous MnCl₂ treatment to excised rice leaves for 24 and 48 h resulted in increased production of H₂O₂ and lipid peroxides, decline in the levels of antioxidants, glutathione and ascorbic acid, and increased activities of antioxidative enzymes, superoxide dismutase, guaiacol peroxidase, catalase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase. Treatment of rice leaves with 100 µM sodium nitroprusside (SNP), a NO donor, was effective in reducing Mn-induced increased levels of H₂O₂, lipid peroxides and increased activities of antioxidative enzymes. The levels of reduced ascorbate and glutathione were considerably recovered due to SNP treatment. The effect of SNP was reversed by the addition of NO scavenger, 2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (c-PTIO) suggesting that ameliorating effect of SNP is due to release of NO. The results indicate that MnCl₂ induces oxidative stress in excised rice leaves, lowers the levels of reduced ascorbate and glutathione, and elevates activities of the key antioxidative enzymes. NO appears to provide a protection to the rice leaves against Mn-induced oxidative stress and that exogenous NO application could be advantageous in combating the deleterious effects of Mn-toxicity in rice plants.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 2
• Opis fizyczny: p.819-825,fig.,ref.

Twórcy

autor

Srivastava S.

• Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India

autor

Dubey R. S.

• Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India

Bibliografia

• Beauchamp CO, Fridovich I (1971) Superoxide dismutase: improved assay and an assay applicable to acrylamide gels. Anal Biochem 44:176–287
• Beers RF, Sizer IW (1952) Colorimetric method for estimation of catalase. J Biol Chem 195:133–139
• Besson-Bard A, Gravot A, Richaud P, Auroy P, Duc C, Gaymard F, Taconnat L, Renou J-P, Pugin A, Wendehenne D (2009) Nitric oxide contributes to cadmium toxicity in Arabidopsis by promoting cadmium accumulation in roots and by up-regulating genes related to iron uptake. Plant Physiol 149:1302–1315
• Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye- binding. Anal Biochem 72:248–254
• Clark D, Dunar J, Navarre DA, Klessig DF (2000) Nitric oxide inhibition of tobacco catalase and ascorbate peroxidase. Mol Plant Microbe Interact 13:1380–1384
• Clemens S (2006) Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie 88:1707–1719
• Courtois C, Besson A, Dahan J, Bourque S, Dobrowolska G, Alain P, Wendehenne D (2008) Nitric oxide signaling in plants: interplays with Ca2⁺ and protein kinases. J Exp Bot 59:155–163
• Demirevska-Kepova K, Simova-Stoilova L, Stoyanova Z, Holzer R, Feller U (2004) Biochemical changes in barley plants after excessive supply of copper and manganese. Environ Exp Bot 52:253–266
• Doulis A, Debian N, Kingston-Smith A, Foyer CH (1997) Characterization of chilling sensitivity in maize: differential localization of antioxidants in maize leaves. Plant Physiol 114:1031–1037
• Egley GH, Paul RN, Vaughn KC, Duke SO (1983) Role of peroxidase in the development of water impermeable seed coats in Sida spinosa L. Planta 157:224–232
• Fecht-Christoffers MM, Maier P, Horst WJ (2003) Apoplastic peroxidase and ascorbate are involved in manganese toxicity and tolerance of Vigna unguiculata. Physiol Plant 117:237–244
• Ferreira LC, Cataneo AC, Ramazzini LMR, Corniani N, Fumis TF, de Souza YA, Scavroni J, Soares BJA (2010) Nitric oxide reduces oxidative stress generated by lactofen in soybean plants. Pesticide Biochem Physiol 97:47–54
• Griffith OW (1980) Determination of glutathione disulphide using glutathione reductase and 2-Vinylpyridine. Anal Biochem 06: 207–212
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I-Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Hsu YT, Kao CH (2004) Cd toxicity is reduced by nitric oxide in rice leaves. Plant Growth Regul 42:227–238
• Innocenti G, Pucciariello C, Gleuher ML, Hopkins J, de Stefano M, Delledonne M, Puppo A, Baudouin E, Frendo P (2007) Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots. Planta 225:1597–1602
• Jana S, Choudhuri MA (1981) Glycolate metabolism of three submerged aquatic angiosperms during aging. Aquat Bot 12: 345–354
• Kopyra M, Gwózdz EA (2003) Nitric oxide stimulates seed germination and counteracts the inhibitory effect of heavy metals and salinity on root growth of Lupinus luteus. Plant Physiol Biochem 41:1011–1017
• Korshunova YO, Eide D, Clark WG, Guerinot ML, Pakrasi HB (1999) The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate range. Plant Mol Biol 40:37–44
• Lamattina L, Garcia-Mata C, Graziano M, Pagnussat G (2003) Nitric oxide: the versatility of an extensive signal molecule. Annu Rev Plant Biol 54:109–136
• Laspina NV, Groppa MD, Tomaro ML, Benavides MP (2005) Nitric oxide protects sunflower leaves against Cd-induced oxidative stress. Plant Sci 169:323–333
• Law MY, Charles SA, Halliwell B (1983) Glutathione and ascorbic acid in spinach (Spinacea oleracea) chloroplasts. The effect of hydrogen peroxide and of paraquat. Biochem J 210:899–903
• Lei Y, Yin C, Ren J, Li C (2007) Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings. Biol Plant 51:386–390
• Lux A, Martinka M, Vaculik M, White PJ (2011) Root responses to cadmium in the rhizosphere—a review. J Exp Bot 62:21–37
• Maheshwari R, Dubey RS (2009) Nickel induced oxidative stress and the role of antioxidant defense in rice seedlings. Plant Growth Regul 59:37–49
• Martinez GR, Mascio PD, Bonini MG, Augusto O, Briviba K, Sies H (2000) Peroxynitrite does not decompose to singlet oxygen (1DO2) and nitroxyl (NO_). Proc Natl Acad Sci USA 97: 10307–10312
• Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
• Nakanishi H, Ogawa I, Ishimaru Y, Mori S, Nishizawa NK (2006) Iron deficiency enhances cadmium uptake and translocation mediated by the Fe²⁺ transporters OsIRT1 and OsIRT2 in rice. Soil Sci Plant Nutr 52:464–469
• Pittman JK (2005) Managing the manganese: molecular mechanisms of manganese transport and homeostasis. New Phytol 167: 733–742
• Sandalio LM, Rodriguez-Serrano M, del Rio LA, Romero-Puettas MC (2009) Reactive oxygen species and signalling in cadmium toxicity. In: del Rio LA, Puppo A (eds) Reactive oxygen species and plant signaling. Springer, Berlin, pp 175–189
• Schaedle M, Bassham JA (1977) Chloroplast glutathione reductase. Plant Physiol 59:1011–1012
• Shah K, Kumar RG, Verma S, Dubey RS (2001) Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Sci 161:1135–1144
• Sharma P, Dubey RS (2007) Involvement of oxidative stress and role of antioxidative defense system in growing rice seedlings exposed to toxic concentrations of aluminium. Plant Cell Rep 26:2027–2038
• Shi QH, Ding F, Wang XF, Wei M (2007) Exogenous nitric oxide protects cucumber roots against oxidative stress induced by salt stress. Plant Physiol Biochem 45:542–550
• Siddiqui MH, Al-Whaibi MH, Basalah MO (2011) Role of nitric oxide in tolerance of plants to abiotic stress. Protoplasma 248:447–455
• Singh HP, Batish DR, Kaur G, Arora K, Kohli RK (2008) Nitric oxide (as sodium nitroprusside) supplementation ameliorates Cd toxicity in hydroponically grown wheat roots. Environ Exp Bot 63:158–167
• Singh HP, Kaur S, Batish DR, Sharma VP, Sharma N, Kohli RK (2009) Nitric oxide alleviates arsenic toxicity by reducing oxidative damage in the roots of Oryza sativa. Nitric Oxide 20:289–297
• Srivastava S, Dubey RS (2011) Manganese-excess induces oxidative stress, lowers the pool of antioxidants and elevates activities of key antioxidative enzymes in rice seedlings. Plant Growth Regul 64:1–16
• Verma S, Dubey RS (2003) Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci 164:645–655
• Wang YS, Yang ZM (2005) Nitric oxide reduces aluminum toxicity by preventing oxidative stress in the roots of Cassia tora L. Plant Cell Physiol 46:1915–1923
• Wendehenne D, Durner J, Klessig DF (2004) Nitric oxide: a new player in plant signalling and defence responses. Curr Opin Plant Biol 7:449–455
• Xiong J, An L, Lu H, Zhu C (2009) Exogenous nitric oxide enhances cadmium tolerance of rice by increasing pectin and hemicellulose contents in root cell wall. Planta 230:755–765
• Xiong J, Fu G, Tao L, Zhu C (2010) Roles of nitric oxide in alleviating effect of heavy metals toxicity in plants. Arch Biochem Biophys 497:13–20
• Xu Y, Sun X, Jin J, Zhou H (2010) Protective effect of nitric oxide on light-induced oxidative damage in leaves of tall fescue. J Plant Physiol 167:512–518
• Yoshida S, Forno DA, Cock JH, Gomez KA (1976) Laboratory manual for physiological studies of rice, 3rd edn. The International Rice Research Institute, Manila
• Yu CC, Hung KT, Kao CH (2005) Nitric oxide reduces Cu toxicity and Cu-induced NH₄⁺ accumulation in rice leaves. J Plant Physiol 162:1319–1330
• Zhao L, He JX, Wang XM, Zhang LX (2008) Nitric oxide protects against polyethylene glycol-induced oxidative damage in two ecotypes of reed suspension cultures. J Plant Physiol 165: 182–191

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