Ganoderma lucidum extract inhibits Schistosoma mansoni survival in silico and in vitro study

• Autorzy: Huseein E.A.M. , Samir M. , Al-Karmalawy A.A. , Hassan S.S. , Embarek Mohamed M. , Mostafa F.A.A. , Moharram A.M. , Alkhalil S.S. , Farrag H.M.M.
• Języki publikacji: EN

Abstrakty

EN

Schistosomosis is a worldwide tropical disease primarily caused by Schistosoma mansoni. Praziquantel is the only available drug for controlling schistosomosis, with many challenges. This study aims to evaluate the in vitro anti-Schistosoma effect of Ganoderma lucidum (G. lucidum) against adult and larval stages of Schistosoma based on the prediction of the binding activity of G. lucidum protein with proteins of various stages of S. mansoni by molecular docking to confirm its inhibitory potential through an insilico study. Results showed that Leu143, Ser165, Met214, and Asn213 were the primary crucial amino acids involved in the binding, with a promising large area of interactions between the two studied proteins. The in vitro study evaluated the motility and survival of adult and larval stages, compared to praziquantel and niclosamide, respectively. There was a significant reduction in the motility of adults after the two-hour incubation, with all concentrations and 100% death of all parasites with the minimal concentration (10 μg/ml) within 4 and 6 h of incubation (P<0.01). Regarding the cercariae, at a concentration of 10 μg/ml, all the cercariae (100%) died (P<0.01) after 15 min, and the miracidial complete mortality rate (100%) (P<0.01) occurred at a concentration of 10 μg/ml after 8 min. This study first predicted the binding activity of G. lucidum protein with proteins of S. mansoni at various stages and proved the anti-Schistosoma effect of G. lucidum in vitro, considered a promising treatment for schistosomosis.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2022
• Tom: 68
• Numer: 2
• Opis fizyczny: p.323-330,fig.,ref.

Twórcy

autor

Huseein E.A.M.

• Department of Medical Parasitology, Faculty of Medicine, Assiut University, Assiut 71516, Egypt

autor

Samir M.

• Department of Medical Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Egypt

autor

Al-Karmalawy A.A.

• Department of Pharmaceutical Medicinal Chemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta 34518, Egypt

autor

Hassan S.S.

• Zoology Department, Faculty of Science, Zagazig University, Egypt

autor

Embarek Mohamed M.

• Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt

autor

Mostafa F.A.A.

• Department of Botany and Microbiology, Faculty of Science, Cairo University, Cairo 12613, Egypt

autor

Moharram A.M.

• Department of Botany, Faculty of Science, Assiut University, Assiut 71515, Egypt

autor

Alkhalil S.S.

• Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Shaqra University, Kingdom of Saudi Arabia

autor

Farrag H.M.M.

• Department of Medical Parasitology, Faculty of Medicine, Assiut University, Assiut 71516, Egypt
• Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Shaqra University, Kingdom of Saudi Arabia

Identyfikatory

DOI

10.17420/ap6802.438