Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding

• Autorzy: Rukke B.A. , Cholidis S. , Johnsen A. , Ottesen P.
• Języki publikacji: EN

Abstrakty

EN

DNA barcoding is a practical tool for species identification, when morphological classification of an organism is difficult. Herein we describe the utilisation of this technique in a case of ophthalmomyiasis interna. A 12-year-old boy was infested during a summer holiday in northern Norway, while visiting an area populated with reindeer. Following medical examination, a Diptera larva was surgically removed from the boy’s eye and tentatively identified from its morphological traits as Hypoderma tarandi (L.) (Diptera: Oestridae). Ultimately, DNA barcoding confirmed this impression. The larval cytochrome c oxidase subunit 1 (COI) DNA sequence was matched with both profiles of five adult H. tarandi from the same region where the boy was infested, and other established profiles of H. tarandi in the Barcode of Life Data Systems (BOLD) identification engine.

Słowa kluczowe

EN

DNA barcoding; species identification; Hypoderma tarandi; Hypoderma bovis; Hypoderma lineatum; Diptera; Oestridae; ophthalmomyiasis; Dermatobia hominis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2014
• Tom: 59
• Numer: 2
• Opis fizyczny: p.301-304,fig.,ref.

Twórcy

autor

Rukke B.A.

• Department of Pest Control, Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway

autor

Cholidis S.

• Department of Ophthalmology, Oslo University Hospital, Kirkeveien 166, 0450 Oslo, Norway

autor

Johnsen A.

• Natural History Museum, University of Oslo, Sars gate 1, 0562 Oslo, Norway

autor

Ottesen P.

• Department of Pest Control, Norwegian Institute of Public Health, Lovisenberggata 8, 0456 Oslo, Norway

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