Genetic diversity of Pyricularia grisea, the causal agent of rice blast by SRR

• Autorzy: Motlagh M.R.S. , Hbibi F. , Ebadi A. A.

Warianty tytułu

PL

Genetyczna różnorodność Pyricularia grisea, czynnika wywołującego zarazę ryżową przez SRR

• Języki publikacji: EN

Abstrakty

EN

Pyricularia grisea, the rice blast fungus is the main pathological threats to rice crop in Iran and worldwide. In this research was evaluated the genetic diversity of P. grisea collected from different fields of Guilan province by using of 14 microsatellite primers. These primers produced 64 polymorphic bands by an average of 4.57 bands for each marker. An average of polymorphic information content in whole primers was 0.734, an average of effective number of alleles was 2.68, an average of Nei’s expected heterozygosity was 0.734 and an average of Shannon’s information index was 1.05. Primer SSR43,44 had the most polymorphic information content (PIC = 0.85), observed number of alleles (na = 8), effective number of alleles (ne = 3.76), Nei’s expected heterozygosity (Ne = 0.861) and Shannon’s information index (I = 1.38). This marker was the best primer between 14 used primers for evaluation the genetic diversity of P. grisea. Cluster analysis was done with simple matching similarity matrix and UPGMA method. The results showed that the studied isolates were classified into 3 lineages by cutting off the dendrogram at 0.76 similar linkage level. Number 1 was the major group and represented most of those isolates. Results of principal coordinate analysis also divided the isolates into three groups exactly similar to obtained with cluster analysis. Overall, our results confirmed that microsatellite primers were good and suitable markers for analyzing structure of P. grisea

PL

Pyricularia grisea, grzyb zarazy ryżowej, jest zagrożeniem dla plonów ryżu w Iranie i na całym świecie. W niniejszym badaniu przy użyciu 14 markerów mikrosateklitarnych oceniano różnorodność genetyczną P. grisea zebranego z różnych pól prowincji Guilan. Markery te tworzyły 64 polimorficznych wiązać przy średnio 4,57 wiązaniach dla każdego markera. ĝrednia informacji polimorficznych we wszystkich markerach wynosiła 0,734, średnia efektywna liczba alleli – 2,68, średnia heterozygotyczność oczekiwana Nei – 0,734 a średni indeks informacji Shannon – 1,05. Marker SSR43,44 miał największą zawartość informacji polimorficznej (PIC = 0.85), największą liczbę alleli obserwowanych (na = 8), liczbę efektywnych alleli (ne = 3.76), największą heterozygotyczność oczekiwaną Nei (Ne = 0,861) i indeks informacji Shannon (I = 1.38). Był to najlepszy spośród 14 markerów użytych do oceny różnorodności genetycznej P. grisea. Analizę skupieć przeprowadzono za pomocą prostego współczynnika simple maching oraz metody UPGMA. Na podstawie wyników badać izolaty zaklasyfikowano do 3 linii przez odcięcie dendogramu na podobnym poziomie powiązać 0,76. Numer 1 był główną grupą i reprezentował większość tych izolatów. Wyniki analizy głównych współrzędnych także dzieliły izolaty na trzy grupy podobne do tych, które otrzymano za pomocą analizy skupieć. Podsumowując, wyniki badać potwierdziły, że markert mikrosatelitarne to dobre i odpowiednie markery do analizy struktury P. grisea.

Słowa kluczowe

EN

genetic diversity; Pyricularia grisea; fungi; rice blast; plant disease; simple sequence repeat

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2015
• Tom: 14
• Numer: 1
• Opis fizyczny: p.15-28,fig.,ref.

Twórcy

autor

Motlagh M.R.S.

• Department of Plant Pathology, Faculty of Agriculture, Islamic Azad University, Rasht Branch, Rasht, Guilan Province PO box 41335-3516, Iran

autor

Hbibi F.

• Department of Plant Pathology, Faculty of Agriculture, Islamic Azad University, Rasht Branch, Rasht, Guilan Province PO box 41335-3516, Iran

autor

Ebadi A. A.

• Rice Research Institute of Iran, Iran

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