Phytochemical screening of Pulsatilla species and investigation of their biological activities

• Autorzy: Laska G. , Sienkiewicz A. , Stocki M. , Zjawiony J.K. , Sharma V. , Bajguz A. , Piotrowska-Niczyporuk A. , Jacob M. , Khan S.
• Języki publikacji: EN

Abstrakty

EN

The present study aimed to identify biologically active secondary metabolites from the rare plant species, Pulsatilla patens subsp. patens and the cultivated P. vulgaris subsp. vulgaris. Chromatographic fractionation of the ethanolic extract of the roots of P. patens subsp. patens resulted in the isolation of two oleanane-type glycosides identified as hederagenin 3-O-β-d-glucopyranoside (2.7 mg) and hederagenin 3-O-β-d-galactopyranosyl-(1→2)-β-d-glucopyranoside (3.3 mg, patensin). HPLC analysis of the methanolic extract of the crude root of P. patens subsp. patens and P. vulgaris subsp. vulgaris revealed the presence of Pulsatilla saponin D (hederagenin 3-O-α-l-rhamnopyranosyl(1→2)-[β-d-glucopyranosyl(1→4)]-α-l-arabinopyranoside). Chromatographic analysis using GC-MS of the silylated methanolic extracts from the leaves and roots of these species identified the presence of carboxylic acids, such as benzoic, caffeic, malic, and succinic acids. The extracts from Pulsatilla species were tested for their antifungal, antimicrobial, and antimalarial activities, and cytotoxicity to mammalian cell lines. Both P. patens subsp. patens and P. vulgaris subsp. vulgaris were active against the fungus Candida glabrata with the half-maximal inhibitory concentration (IC₅₀) values of 9.37 μg/mL and 11 μg/mL, respectively. The IC₅₀ values for cytotoxicity evaluation were in the range of 32–38 μg/mL for P. patens subsp. patens and 35–57 μg/mL for P. vulgaris subsp. vulgaris for each cell line, indicating general cytotoxic activity throughout the panel of evaluated cancer and noncancer cells.

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2019
• Tom: 88
• Numer: 1
• Opis fizyczny: Article 3613 [17p.],fig.,ref.

Twórcy

autor

Laska G.

• Department of Agri-Food Engineering and Environmental Management, Bialystok University of Technology, Wiejska 45A, 15-351 Bialystok, Poland

autor

Sienkiewicz A.

• Department of Agri-Food Engineering and Environmental Management, Bialystok University of Technology, Wiejska 45A, 15-351 Bialystok, Poland

autor

Stocki M.

• Department of Forestry in Hajnówka, Bialystok University of Technology, Wiejska 45A, 15-351 Bialystok, Poland

autor

Zjawiony J.K.

• Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, MS 38677, United States

autor

Sharma V.

• Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, MS 38677, United States

autor

Bajguz A.

• Department of Plant Biochemistry and Toxicology, University of Bialystok, Ciolkowskiego 1J, 15-245 Bialystok, Poland

autor

Piotrowska-Niczyporuk A.

• Department of Plant Biochemistry and Toxicology, University of Bialystok, Ciolkowskiego 1J, 15-245 Bialystok, Poland

autor

Jacob M.

• National Center for Natural Products Research, University of Mississippi, University, MS 38677, United States

autor

Khan S.

• National Center for Natural Products Research, University of Mississippi, University, MS 38677, United States

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Identyfikatory

DOI

10.5586/asbp.3613