Heterologous expression of Populus euphratica CPD (PeCPD) can repair the phenotype abnormity caused by inactivated AtCPD through restoring brassinosteroids biosynthesis in Arabidopsis

• Autorzy: Wu H. , Si J. , Xu D. , Lian G. , Wang X.
• Języki publikacji: EN

Abstrakty

EN

To get insights into the functional difference of CPD (constitutive photomorphogenesis and dwarfism) between herb and woody plants, a full-length Populus euphratica L. cDNA homologous to Arabidopsis thaliana CPD (AtCPD), named PeCPD, was introduced to Arabidopsis thaliana cpd mutant (CM) and corresponding wild type (WT), resulting in a series of CM-PeCPD and WT-PeCPD transgenic lines. All the CM-PeCPD lines differentially displayed evident restoration in phenotype and fertility compared to cpd mutant, but still showed differences from WT in some respects. All the WT-PeCPD lines displayed obvious overexpression phenotype compared to WT plants. The transcription levels (TLs) of PeCPD in the CM-PeCPD lines were positively correlated, and that in the WT-PeCPD lines uncorrelated, with the level of their phenotype restoration/change. In the CM-PeCPD lines, the TLs of AtDWF4, AtBR6OX2 and AtTCH4 were negatively, and of AtBAS1 and AtSAUR-AC1 positively correlated with PeCPD TLs, whereas in the WT-PeCPD lines, their TLs were uncorrelated, and positively or negatively correlated to PeCPD TLs. The level of total endogenous BRs was basically negatively correlated to the level of phenotype restoration/change and PeCPD TLs in the PeCPD transgenic plants. The findings indicate that PeCPD also plays important role in regulation of plant growth and development through participating in BR biosynthesis.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 12
• Opis fizyczny: p.3123-3135,fig.,ref.

Twórcy

autor

Wu H.

• Key Laboratory of Cell Activities and Stress Adaptation of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, 730000, People’s Republic of China

autor

Si J.

• Key Laboratory of Cell Activities and Stress Adaptation of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, 730000, People’s Republic of China

autor

Xu D.

• Key Laboratory of Cell Activities and Stress Adaptation of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, 730000, People’s Republic of China

autor

Lian G.

• Key Laboratory of Cell Activities and Stress Adaptation of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, 730000, People’s Republic of China

autor

Wang X.

• Key Laboratory of Cell Activities and Stress Adaptation of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, 730000, People’s Republic of China

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Identyfikatory

DOI

10.1007/s11738-014-1633-6