Effect of cadmium on DNA changes in Ipomoea aquatica Forssk

• Autorzy: Tanee T. , Sudmoon R. , Thamsenanupap P. , Chaveerach A.
• Języki publikacji: EN

Abstrakty

EN

Cadmium (Cd) contamination in plants fertilized with inorganic fertilizers is a major risk to human health. Ipomoea aquatica Forssk., which is one of the most popular vegetables used in Thai cuisine, is also vulnerable to Cd contamination. This study aimed to investigate Cd accumulation in Ipomoea aquatica Forssk. and the associated changes in its DNA. The plant was grown in soil supplemented with Cd at 0, 15, 30, 60, and 120 mg/kg. After 21 days, accumulation in the roots, stems, and leaves was analyzed using atomic absorption spectrophotometry (AAS), and the bioconcentration (BCF) and translocation factors (TF) were analyzed. DNA changes were assessed by a combination of random amplified polymorphic DNA (RAPD) and genomic template stability (GTS) tests. Cd concentrations in the roots, stems, and leaves ranged from 0 to 12,333 mg/kg, 0 to 5,909.27 mg/kg, and 0 to 1,653.26 mg/kg, respectively. The BCF and TF values ranged from 0 to 21.15 and 0 to 1.21, respectively. From the RAPD profiles, the GTS values ranged from 52.3 to 91.1%. Taken together, these results indicate that I. aquatica is a Cd-hyperaccumulator; therefore, consuming I. aquatica plants grown in Cd-polluted areas is a health risk.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2016
• Tom: 25
• Numer: 1
• Opis fizyczny: p.311-315,fig.,ref.

Twórcy

autor

Tanee T.

• Faculty of Environment and Resource Studies, Mahasarakham University, Maha Sarakham 44150, Thailand
• Genetics and Environmental Toxicology (GET) Research Group, Khon Kaen University, Khon Kaen 40002, Thailand

autor

Sudmoon R.

• Genetics and Environmental Toxicology (GET) Research Group, Khon Kaen University, Khon Kaen 40002, Thailand
• Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand

autor

Thamsenanupap P.

• Faculty of Environment and Resource Studies, Mahasarakham University, Maha Sarakham 44150, Thailand

autor

Chaveerach A.

• Genetics and Environmental Toxicology (GET) Research Group, Khon Kaen University, Khon Kaen 40002, Thailand
• Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand

Bibliografia

• 1. MANAHAN S.E. Toxicological Chemistry Biochemistry. CRC Press; 449, USA, 2003.
• 2.MIURA N., ASHIMORI A., TAKEUCHI A., OHTANI K., TAKADA N., YANAGIBA Y., MITA M., TOGAWA M., HASEGAWA T. Mechanisms of cadmium-induced chronotoxicity in mice. J. Toxicol. Sci., 38 (6), 947, 2013.
• 3. WHO. Evaluation of certain food additives and contaminants: forty-first report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Technical Report Series; 837, 1993.
• 4. MINISTRY OF PUBLIC HEALTH, THAILAND. Announcement of Ministry of Public Health No. 98 (1986), Standard Level on Contaminants in Foods, 1986.
• 5. THE COMMISSION OF THE EUROPEAN COMMUNITIES. Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union, 364, 24, 2006.
• 6. LIU W., LI P.J., QI X.M., ZHOU Q.X., ZHENG L., SUN T.H., YANG Y.S. DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis. Chemosphere, 61, 158, 2005.
• 7. ATIENZAR F.A., CONRADI M., EVENDEN A.J., JHA A.N., DEPLEDGE M.H. Qualitative assessment of genotoxicity using random amplified polymorphic DNA: comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene. Environ. Toxicol. Chem., 18 (10), 2275, 1999.
• 8. LIU W., YANG Y.S., LI P.J., ZHOU Q.X., XIE L.J., HAN Y.P. Risk assessment of cadmium-contaminated soil on plant DNA damage using RAPD and physiological indices. J. Hazard. Mater., 161, 878, 2009.
• 9. LIU W., YANG Y.S., ZHOU Q., XIE L., LI P., SUN T. Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers. Chemosphere, 67, 1155, 2007.
• 10. DUMAN D.C., ALTUNKAYNAK E., ARAS, E.S. Heavy metal accumulation and genotoxicity indicator capacity of the lichen species Ramalina pollinaria collected from around an iron steel factory in Karabük, Turkey. Turk. J. Bot., 38, 14, 2014.
• 11. BOONMEE S., NEERATANAPHAN L., TANEE T., KHAMON P. The genetic differentiation of Colocasia esculenta growing in gold mining areas with arsenic contamination. Environ. Monit. Assess., 187, 227, 2015.
• 12. TANEE T., CHAVEERACH A., NARONG C., PIMJAI M., PUNSOMBUT P., SUDMOON R. Bioaccumulation of heavy metals in fish from the Chi River, Maha Sarakham province, Thailand. Int. J. Biosci., 3, 159, 2013.
• 13. POREBSKI S., BAILEY L.G., BAUM B.R. Modification of a CTAB extraction protocol for plants containing high polysaccharides and polyphenol component. Plant Mol. Biol. Report., 15, 8, 1997.
• 14. MALIK R.N., HUSAIN S.Z., NAZIR I. Heavy metal contamination and accumulation in soil and wild plant species from industrial area of Islamabad, Pakistan. Pakistan J. Bot., 42, (1), 291, 2010.
• 15. SUN Y., ZHOU Q., DIAO C. Effects of cadmium and arsenic on growth and metal accumulation of Cd-hyperaccumulator Solanum nigrum L. Bioresour. Technol., 99, 1103, 2008.
• 16. SUN Y., ZHOU Q., WANG L., LIU W. Cadmium tolerance and accumulation characteristics of Biden spilosa L. as a potential Cd-hyperaccumulator. J. Hazard. Mater., 161, 808, 2009.
• 17. LORESTANI B., CHERAGHI M., YOUSEFI N. Phytoremediation potential of native plants growing on a heavy metals contaminated soil of copper mine in Iran. World Acad. Sci. Eng. Technol. 5, 365, 2011.
• 18. SUDMOON R., NEERATANAPHAN L., THAMSENANUPAP P., TANEE T. Hyperaccumulation of cadmiumand DNA changes in popular vegetable, Brassica chinensis L. Int. J. Environ. Res., 9 (2), 433, 2015.
• 19. QIN T., WU Y., WANG H. Effect of cadmium, lead and their interactions on the physiological and biochemical characteristics of Brassica chinensis. Acta Ecol. Sin., 14, 46, 1994.
• 20. YAN S., LING Q., BAO Z., CHEN Z., YAN S., DONG Z., ZHANG B., DENG B. Cadmium accumulation in pakchoi (Brassica chinensis L.) and estimated dietary intake in the suburb of Hangzhou city, China. Food Addit. Contam. Part B Surveill., 2, 74, 2009.

Identyfikatory

DOI

10.15244/pjoes/60726