Axenic cultivation and comparative phospholipase A2 activity of Giardia duodenalis in a serum-free medium

• Autorzy: Mata-Cardenas B.D. , Hernandez-Garcia M. E. , Gonzalez-Salazar F. , Garza-Gonzalez J. N. , Palacios-Corona R. , Cortes-Gutierrez E. I. , Vargas-Villarrewal J.
• Języki publikacji: EN

Abstrakty

EN

Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle’s amino acid solution, Diamond’s vitamin-tween 80 mixtures and LCR (a lipid-cholesterol — rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A2 (PLA A2) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95% compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A2 activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum.

Słowa kluczowe

EN

Giardia duodenalis; culture medium; bovine serum; phospholipase A2; Entamoeba histolytica; Trichomonas vaginalis; axenic cultivation; trophozoite

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2012
• Tom: 57
• Numer: 3
• Opis fizyczny: p.211-215,ref.

Twórcy

autor

Mata-Cardenas B.D.

• Facultad de Ciencias Quimicas, Universidad Autonoma de Nuevo Leon, Av. Pedro de Alba y Manuel L. Barragan s/n, Cd. Universitaria, C.P. 66451, San Nicolas de los Garza, Nuevo Leon, Mexico

autor

Hernandez-Garcia M. E.

autor

Gonzalez-Salazar F.

autor

Garza-Gonzalez J. N.

autor

Palacios-Corona R.

autor

Cortes-Gutierrez E. I.

autor

Vargas-Villarrewal J.

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