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2013 | 57 | 3 |

Tytuł artykułu

Expression of CD3, CD4, CD8, CD21, and MHC II lymphocyte antigens and serum IL-10 concentration in dogs with atopic dermatitis complicated by purulent dermatitis

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Dog blood samples were analysed cytometrically and immunoenzymatically. In dogs with atopic dermatitis complicated by purulent dermatitis, the percentage of lymphocytes with CD8, CD21 antigens, as well as IL-10 concentration in serum were significantly higher than in healthy dogs. The results indicate that immunosuppression induced by the activity of an infectious agent plays an important role at this stage of the disease. This thesis was further confirmed by a high positive coiTelation between MHC II CD3, CD4, and CD21 expression and a negative correlation between cells with MHC II and CD8 lymphocytes.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

57

Numer

3

Opis fizyczny

p.365-370,fig.,ref.

Twórcy

autor
  • Sub-department of Clinical Diagnostic and Veterinary Dermatology, Department and Clinic of Internal Medicine, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland

Bibliografia

  • 1. Antunez C., Torres M.J., Mayorga C.: Different cytokine production and activation marker profiles in circulating cutaneus-lymphocyte- associated antigen + T cells from patients with acute or chronic atopic dermatitis. Clin Exp Allergy 2004, 34, 559-566.
  • 2. Byrne K.M., Hong Wook K., Chew BP., Reinhard G.A., Hayek M.G.: A standardized gating technique for generation of flow cytometry data for normal canine and normal feline blood lymphocytes. Vet Immunol Immunopathol 2000, 73, 167-184.
  • 3. Chabanne L., Marchal T., Denerolle P., Magnol J.P., Foumel C., Monier J.C., Rigal D.: Lymphocyte subset abnormalities in German shepherd dog pyoderma (GSP). Vet Immunol Immunopathol 1995, 49, 189-198.
  • 4. Chabanne L., Bonnefont C., Bernaud J., Rigal D.: Clinical application of flow cytometry and cell immunophenotyping to companion animals (dog and cat). Methods Cell Sci 2000, 22, 199-207.
  • 5. Dzienis K., Tryniszewska E., Kaczmarski M.: Disorders of immunological balance between Th1 and Th2 cells and the role of chosen cytokines in atopic dermatitis. Post Dermatol Alergol 2006, 2, 88-93.
  • 6. Faldyna M., Leva L., Knotigova P., Toman M.: Lymphocyte subsets in peripheral blood of dogs- a flow cytometric study. Vet Immunol Immunopathol 2001, 82, 23-37.
  • 7. Favrot C., Steffan J., Seewald W., Picco F.: A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis. Vet Dermatol 2010, 21, 23-31.
  • 8. Fiorentini S., Licenziati S., Alessandri G., Castelli F., Caligaris S., Bonafede M., Grassi M., Garrafa E., Balsari A., Turano A., Caruso A.: CD11b expression identifies CD8+CD28+ Y lymphocytes with phenotype and function of both naive/memory and effector cells. J Immunol 2001, 166, 900- 907.
  • 9. Keppel K.E., Campbell K.L., Zuckermann F.A., Greeley E.A., Schaeffer D.J., Husmann R.J.: Quantitation of canine regulatory T cell populations, serum interleukin-10 and allergen-specific IgE concentrations in healthy control dogs and canine atopic dermatitis patients receiving allergen- specific immunotherapy. Vet Immunol Immunother 2008, 123, 337-344.
  • 10. Machura E., Karczewska K., Mazur B., Jachimowicz M.: Serum levels of IL-2, IL-10, IL-13, IFNγ and TNFα in children with asthma and atopic dermatitis (AD). Allerg Astma Immun 2008, 13, 172-181.
  • 11. Marsella R., Olivry T., Nicklin C., Lopez J.: Pilot investigation of model for canine atopic dermatitis: environmental house dust mite challenge of high-IgE-producing beagles, mite hypersensitive dogs with atopic dermatitis and normal dogs. Vet Dermatol 2006, 17, 24-35.
  • 12. Merryman-Simpson A.E., Wood S.H., Fretwell N., Jones P.G., McLaren W.M., McEwan N., Clements D.N., Carter S.D., Ollier W.E., Nuttal S.D.: Gene (nRNA) expression in canine atopic dermatitis: microarray analysis. Vet Dermatol 2008, 19, 59-66.
  • 13. Olivry T., DeBoer D.J., Favrot C., Jackson H.A., Mueller R.S., Nuttal T., Prelaud P.: Treatment of canine atopic dermatitis: 2010 clinical practice guidelines from the International Task Force on Canine Atopic Dermatitis. Vet Dermatol 2010, 21, 233-248.
  • 14. Ricklin M.E., Roosje P., Summerfield A.: Characterization of the canine dendritic cells in healthy, atopic and non-allergic inflamed skin. J Clin Immunol 2010, 30, 845-854.
  • 15. Reis A.B., Carneiro C.M., Carvahlo M.G., Teixeira Carvahlo A., Giunchetti R., Mayring W., Genaro O., Correa-Oliveira R.: Establishment of a microplate assay for flow cytometric assessment and it is use for the evaluation of age-related phenotypic changes in canine whole blood leukocytes. Vet Immunol Immunopathol 2005, 103, 173-185.
  • 16. Roosje P.: Canine atopic dermatitis: New concept. Eur J Comp Animal Pract 2005, 15, 189-195.
  • 17. Schlotter Y.M., Rutten V.P.M.G., Riemers F.M., Knol E.F., Willemse T.: Lesional skin in atopic dogs shows a mixed Type 1 and Type 2 immune responsiveness. Vet Immunol Immunopathol 2011, 143, 20-26.
  • 18. Silny W., Czarnecka-Operacz M., Glinski W., Samochocki Z., Jenerowicz D.: Atopic dermatitis contemporary view on pathomechanism and management. Position statement of Polish Dermatological Society specialists. Post Dermatol Allergol 2010, 27, 365-382.
  • 19. Stasiak- Barmuta A., Stankiewicz W., Chciałowski A., Moniuszko T., Alifier M.: Concentration of IL-10, IL-13 and IFNγ cytokines in supematants of microcultured CD4+ and NK cells in patients with non allergie bronchial asthma. Pol Merkuriusz Lek 2008, 139, 8-13.
  • 20. Uchida T., Suto H., Ra C., Ogawa H., Kobata T., Okumura K.: Preferential expression of Th2-type chemokine and its receptor in atopic dermatitis. Int Immunol 2002, 14, 1431-1438.
  • 21. Watabe A., Fukumoto S., Komatsu T., Endo Y., Kadosawa T.: Alterations of lymphocyte subpopulations in healthy dogs with agile and in dogs with cancer. Vet Immunol Immunopathol 2011, 142, 189-200.

Typ dokumentu

Bibliografia

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