Role of adaptor complex AP2 in formation of dendritic arbors of hippocampal neurons

• Autorzy: Koscielny A. , Malik A. , Tempes A. , Jaworski J.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: The proper functioning of neurons is defined by many different aspects such as dendritic branching and receptor composition within synapses. Abnormalities in dendritic tree development lead to serious dysfunctions of the nervous system. Not surprisingly, the multi-step process of dendritic tree development is highly regulated. In shRNA library screen we have identified several proteins as modifiers of dendritic tree, also involved in various aspects of membrane trafficking. b-adaptin, a key component of AP2 adaptor complex, indispensable for clathrindependent endocytosis was one of them. Thus, the aim of this study was to find how exactly AP2 complex contributes to shaping dendritic arbor of hippocampal neurons. METHODS: To study role ofAP2 complex in dendritic arborization of hippocampal neurons we used primary hippocampal neurons expressing shRNA against subunits of AP2 complex alone or in combination of functional rescue constructs (e.g. shRNA-resistant AP2 subunits, mutants of GluA2 subunit). RESULTS: We show that knockdown of a and b subunits of AP2 complex as well as clathrin lead to severe reduction in number of dendrites of developing hippocampal neurons. We also show that this negative effect of b-adaptin knockdown can be rescued by overexpression of GluA2, a prototypical AP2 cargo in neurons. It was, however, possible only when ion channel GluA2 was left intact. Our preliminary data from Western blot and immunofluorescence experiments, imply that in developing neurons knockdown of AP2 leads to abnormal processing and degradation of GluA2 what eventually may lead to dendritic arbor underdevelopment. CONCLUSIONS: In developing hippocampal neurons b-adaptin is needed for proper growth of dendritic arbors, most likely by ensuring proper processing of surface receptors needed for this process. This work has been financed by National Research Centre grant no. 2011/03/B/NZ3/01970.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S64

Twórcy

autor

Koscielny A.

• International Institute of Molecular and Cell Biology in Warsaw, Warsaw, Poland

autor

Malik A.

• International Institute of Molecular and Cell Biology in Warsaw, Warsaw, Poland

autor

Tempes A.

• International Institute of Molecular and Cell Biology in Warsaw, Warsaw, Poland

autor

Jaworski J.

• International Institute of Molecular and Cell Biology in Warsaw, Warsaw, Poland