Pathogenicity and ultrastructural studies of the mode of penetration by Phyllosticta plantaginis in ribwort leaves

• Autorzy: Zimowska B.

Warianty tytułu

PL

Patogeniczność i ultrastrukturalne badania nad modelem infekcji liści babki lancetowatej przez Phyllosticta plantaginis

• Języki publikacji: EN

Abstrakty

EN

Pathogenicity and ultrastructural investigation of the ribwort leaves inoculated with Phyllosticta plantaginis conidia was undertaken using scanning electron microscopy to examine the host-parasite relationship. Pathogenicity experiments demonstrated that all tested P. plantaginis isolates had infected leaves of ribwort. Of all inoculation methods, direct placement of colonized agar plugs on damaged epidermis and soaking leaves in conidial suspension were the most effective. The behavior of the conidia deposited on the leaves was investigated at different time intervals after inoculation: 7, 18, 25, 48 and 72 h. An appressorium appeared directly at the end of a short germ tube grown from conidia. Appressoria were formed over the cuticle in some distance from the stomata. Penetration through the stomata was observed.

PL

W pracy podjęto badania nad spełnieniem postulatów Kocha dla pięciu izolatów Phyllosticta plantaginis oraz ultrastrukturą inokulowanych liści babki w celu wyjaśnienia powinowactwa pomiędzy P. plantaginis a rośliną żywicielską. Badania wykazały, że wszystkie testowane izolaty spowodowały infekcję na inokulowanych liściach babki. Z trzech uwzględnianych w badaniach metod inokulacji, najbardziej efektywnymi okazały się metoda polegająca na umieszczaniu krążków pożywki przerośniętej przez grzybnię na zranionej tkance liścia oraz metoda uwzględniająca zanurzenie liści w zawiesinie zarodników konidialnych. Obserwacje ultrastruktury inokulowanych liści babki prowadzono w mikroskopie skaningowym po 7, 18, 25, 48 oraz 72 godzinach. Wykazały one tworzenie się appresorium na końcu strzępki kiełkowej oraz wnikanie patogenu przez aparaty szparkowe.

Słowa kluczowe

EN

pathogenicity; ultrastructure; penetration; Phyllosticta plantaginis; ribwort zob.small plantain; small plantain; ribwort plantain zob.small plantain; English plantain zob.small plantain; narrowleaf plantain zob.small plantain; ribleaf zob.small plantain; buckhorn zob.small plantain; buckhorn plantain zob.small plantain; lamb's tongue zob.small plantain; herb; leaf; plant infection; infection process

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2014
• Tom: 13
• Numer: 3
• Opis fizyczny: p.143-153,fig.,ref.

Twórcy

autor

Zimowska B.

• Department of Plant Pathology and Mycology, University of Life Sciences in Lublin, Leszczynskiego 7, 20-069 Lublin, Poland

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