Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method

• Autorzy: Gryszczynska A. , Krajewska-Patan A. , Buchwald W. , Czerny B. , Mielcarek S. , Rudzinska K. , Mroziewicz P. M.

Warianty tytułu

PL

Porównanie zawartości proantocyjanidyn w korzeniach Rhodiola kirilowii i Rhodiola rosea z wykorzystaniem metody UPLC-MS/MS

• Języki publikacji: EN

Abstrakty

EN

The purpose of presented study was the comparison of flavan-3-ol content in the roots of Rhodiola kirilowii and Rhodiola rosea with use of UPLC-MS/MS method. Two kinds of extract were prepared: aqueous extract and 50% v/v ethanol extract. The worked out UPLC MS/MS method allowed to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). The obtained results shown that the content of measured catechins was higher in R. kirilowii roots than in R. rosea. Both Rhodiola roots contain EGCG as a main proanthocyanidin compound – the content in R. kirilowii roots is about 0.14%. Our results indicate that application of ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) allows to determine the proanthocyanidins content in tested samples with satisfactory precision and can be used in the Rhodiola sp. phytochemical investigations.

PL

Celem przedstawionych badań było porównanie za pomocą opracowanej metodyki wykorzystującej ultrasprawny chromatograf cieczowy sprzężony z tandemowym spektrometrem mas (Waters) zawartości flawan-3-oli w korzeniach Rhodiola kirilowii i Rhodiola rosea. Badano wodne i alkoholowo-wodne (50% EtOH) wyciągi z korzeni. Opracowana metoda UPLC MS/MS pozwoliła na określenie stężeń pięciu flawan-3-oli: (+)-katechiny, (-)-epikatechiny, (-)-epigalokatechiny, galusanu (-)-epikatechiny (ECG) oraz galusanu (-)-epigalokatechiny (EGCG). Otrzymane wyniki wskazują, że zawartość tych katechin jest wyższa w korzeniach R. kirilowii niż w korzeniach R. rosea. Oba surowce zawierają galusan (-)-epigallokatechiny jako główną proantocyjanidynę – jej zawartość w korzeniach R. kirilowii wynosi ok. 0,14%. Uzyskane przez nas wyniki wskazują, że metoda analityczna z wykorzystaniem ultrasprawnego chromatografu cieczowego sprzężonego z tandemowym spektrometrem mas pozwala z zadawalającą precyzją oznaczyć zawartości proantocyjanidyn w analizowanych próbkach i może być stosowana w badaniach rodzaju Rhodiola.

Słowa kluczowe

EN

comparison; proanthocyanidin; proanthocyanidin content; Rhodiola kirilowii; Rhodiola rosea; root; application; ultra performance liquid chromatography-tandem mass spectrometric method; UPLC-MS-MS method zob.ultra performance liquid chromatography-tandem mass spectrometric method

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2012
• Tom: 58
• Numer: 3
• Opis fizyczny: p.5-15,fig.,ref.

Twórcy

autor

Gryszczynska A.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Insitute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Krajewska-Patan A.

autor

Buchwald W.

autor

Czerny B.

autor

Mielcarek S.

autor

Rudzinska K.

autor

Mroziewicz P. M.

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