DNA vaccine encoding Haemonchus contortus Actin induces partial protection in Goats

• Autorzy: Yan R. , Wang J. , Xu L. , Song X. , Li X.
• Języki publikacji: EN

Abstrakty

EN

Actin is a globular multi-functional protein that forms microfilaments, and participates in many important cellular processes. Previous study found that Haemonchus contortus actin could be recognized by the serum of goats infected with the homology parasite. This indicated that H. contortus actin could be a potential candidate for vaccine. In this study, DNA vaccine encoding H. contortus actin was tested for protection against experimental H. contortus infections in goats. Fifteen goats were allocated into three trial groups. The animals of Actin group were vaccinated with the DNA vaccine on day 0 and 14, and challenged with 5000 infective H. contortus third stage larval (L3) on day 28. An unvaccinated positive control group was challenged with L3 at the same time. An unvaccinated negative control group was not challenged with L3. The results showed that DNA vaccine were transcribed at local injection sites and expressed in vivo post immunizations respectively. For goats in Actin vaccinated group, higher levels of serum IgG, serum IgA and mucosal IgA were produced, the percentages of CD4+ T lymphocytes, CD8+ T lymphocytes and B lymphocytes and the concentrations of TGF-β were increased significantly (P<0.05). Following L3 challenge, the mean eggs per gram feces (EPG) and worm burdens of Actin group were reduced by 34.4% and 33.1%, respectively. This study suggest that recombinant H. contortus Actin DNA vaccine induced partial immune response and has protective potential against goat haemonchosis.

Słowa kluczowe

EN

DNA vaccine; muscle cell; Haemonchus contortus; actin; multifunctional protein; goat; infection; flow cytometry

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2014
• Tom: 59
• Numer: 4
• Opis fizyczny: p.698-709,fig.,ref.

Twórcy

autor

Yan R.

• College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

autor

Wang J.

• College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

autor

Xu L.

• College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

autor

Song X.

• College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

autor

Li X.

• College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Bibliografia

• Alarcon J.B., Waine G.W., McManus D.P. 1999. DNA vaccines: technology and application as anti-parasite and anti-microbial agents. Advances in Parasitology, 42, 343–410. DOI: http://dx.doi.org/ 10.1016/S0065-308X(08)60152-9.
• Amarante A.F., Bricarello P.A., Huntley J.F., Mazzolin L.P., Gomes J.C. 2005. Relationship of abomasal histology and parasite-specific immunoglobulin A with the resistance to Haemonchus contortus infection in three breeds of sheep. Veterinary Parasitology, 128, 99–107. DOI: http://dx.doi.org/ 10.1016/j.vetpar.2004.11.021.
• Balic A., Bowles V.M., Meeusen E.N. 2000. Cellular profiles in the abomasal mucosa and lymph node during primary infection with Haemonchus contortus in sheep. Veterinary Immunology and Immunopathology, 75, 109–120. DOI: http://dx.doi.org/ 10.1016/S0165-2427(00)00189-6.
• Barger I.A. 1993. Influence of sex and reproductive status on susceptibility of ruminants to nematode parasitism. International Journal for Parasitology, 23, 463–469. DOI: http://dx.doi.org/ 10.1016/0020-7519(93)90034-V.
• Butterworth A.E. 1985. Cell-mediated damage to helminths. Advances in Parasitology, 23, 143–235. DOI: http://dx.doi.org/ 10.1016/S0065-308X(08)60287-0.
• Chen N., Yuan Z.G., Xu M.J., Zhou D.H., Zhang X.X., Zhang Y.Z., Wang X.W., Yan C., Lin R.Q., Zhu X.Q. 2012. Ascaris suum enolase is a potential vaccine candidate against ascariasis. Vaccine, 30, 3478–3482. DOI: http://dx.doi.org/ 10.1016/j.vaccine.2012.02.075.
• Domńguez-Toraño I.A., Fernández-Pérez F.J., Gómez-Muñoz M.T., Alunda J.M., Cuquerella M. 2003. Humoral and cellular response in lambs vaccinated against Haemonchus contortus with p26/23. Small Ruminant Research, 50, 29–37. DOI: http://dx.doi.org/ 10.1016/S0921-4488(03)00105-6.
• Geldhof P., Claerebout E., Knox D., Vercauteren I., Looszova A., Vercruysse J. 2002. Vaccination of calves against Ostertagia ostertagi with cysteine proteinase enriched protein fractions. Parasite Immunology, 24, 263–270. DOI: http://dx.doi.org/ 10.1046/j.1365-3024.2002.00461.x.
• Gill H.S., Watson D.L., Brandon M.R. 1993. Monoclonal antibody to CD4+ T cells abrogates genetic resistance to Haemonchus contortus in sheep. Immunology, 78, 43–49.
• Han K., Xu L., Yan R., Song X., Li X. 2012. Vaccination of goats with glyceraldehyde-3-phosphate dehydrogenase DNA vaccine induced partial protection against Haemonchus contortus. Veterinary Immunology and Immunopathology, 149, 177–185. DOI: http://dx.doi.org/ 10.1016/j.vetimm.2012.06.016.
• Herd R.P., Queen W.G., Majewski G.A. 1992. Sex-related susceptibility of bulls to gastrointestinal parasites. Veterinary Parasitology, 44, 119–125. DOI: http://dx.doi.org/ 10.1016/0304-4017(92)90150-8.
• Huntley J.F., Patterson M., Mackellar A., Jackson F., Stevenson L.M., Coop R.L. 1995. A comparison of the mast cell and eosinophil responses of sheep and goats to gastrointestinal nematode infections. Research in Veterinary Science, 58, 5–10. DOI: http://dx.doi.org/ 10.1016/0034-5288(95)90080-2.
• Karanu F.N., McGuire T.C., Davis W.C., Besser T.E., Jasmer D.P. 1997. CD4+ T lymphocytes contribute to protective immunity induced in sheep and goats by Haemonchus contortus gut antigens. Parasite Immunology, 19, 435–445. DOI: http://dx.doi.org/ 10.1046/j.1365-3024.1997.d01-149.x.
• Knox D.P., Redmond D.L., Jones D.G. 1993. Characterization of proteinases in extracts of adult Haemonchus contortus, the ovine abomasal nematode. Parasitology, 106, 395–404. DOI: http://dx.doi.org/ 10.1017/S0031182000067147.
• Knox D.P., Smith W.D. 2001. Vaccination against gastrointestinal nematode parasites of ruminants using gut-expressed antigens. Veterinary Parasitology, 100, 21–32. DOI: http://dx.doi.org/ 10.1016/S0304-4017(01)00480-0.
• Masih S., Arora S.K., Vasishta R.K. 2011. Efficacy of Leishmania donovani ribosomal P1 gene as DNA vaccine in experimental visceral leishmaniasis. Experiment Parasitology, 129, 55–64. DOI: http://dx.doi.org/ 10.1016/j.exppara.2011.05.014.
• Meeusen E.N., Balic A. 2000. Do eosinophils have a role in the killing of helminth parasites? Parasitol Today, 16, 95–101. DOI: http://dx.doi.org/ 10.1016/S0169-4758(99)01607-5.
• Molina J.M., Martín S., Hernández Y.I., González J.F., Ferrer O., Ruiz A. 2012. Immunoprotective effect of cysteine proteinase fractions from two Haemonchus contortus strains adapted to sheep and goats. Veterinary Parasitology, 188, 53–59. DOI: http://dx.doi.org/ 10.1016/j.vetpar.2012.03.010.
• Muleke C.I., Ruofeng Y., Yanming S., Guangwei Z., Lixin X., Xiangrui L. 2007a. Vaccination of goats against Haemonchus contortus with a recombinant cysteine protease. Small Ruminant Research, 73, 95–102. DOI: http://dx.doi.org/ 10.1016/j.smallrumres.2006.11.006.
• Muleke C.I., Ruofeng Y., Lixin X., Xinwen B., Xiangrui L. 2007b. Cloning and sequence analysis of Hemonchus contortus HC58cDNA. DNA Sequence, 18, 176–183.
• Munn E.A., Smith T.S., Graham M., Greenwood C.A., Tavernor A.S., Coetzee G. 1993a. Vaccination of merino lambs against haemonchosis with membrane-associated proteins from the adult parasite. Parasitology, 106, 63–66. DOI: http://dx.doi.org/ 10.1017/S0031182000074825.
• Munn E.A., Smith T.S., Graham M., Tavernor A.S., Greenwood C.A. 1993b. The potential value of integral membrane proteins in the vaccination of lambs against Haemonchus contortus. International Journal for Parasitology, 23, 261–269. DOI: http://dx.doi.org/ 10.1016/0020-7519(93)90149-S.
• Newlands G.F., Skuce P.J., Knox D.P., Smith W.D. 2001. Cloning and expression of cystatin, a potent cysteine protease inhibitor from the gut of Haemonchus contortus. Parasitology, 122, 371–378. DOI: http://dx.doi.org/ 10.1017/S0031182001007302/.
• Newton S.E., Munn E.A. 1999. The development of vaccines against gastrointestinal nematode parasites, particularly Haemonchus contortus. Parasitology Today, 15, 116–122. DOI: http://dx.doi.org/ 10.1016/S0169-4758(99)01399-X/.
• Pfeffer A., Douch P.G., Shaw R.J., Gatehouse T.K., Rabel B., Green R.S., Shirer C.L., Jonas W.E., Bisset S. 1996. Sequential cellular and humoral responses in the abomasal mucosa and blood of Romney sheep dosed with Trichostrongylus axei. International Journal for Parasitology, 26, 765–773. DOI: http://dx.doi.org/ 10.1016/0020-7519(96)00052-5/.
• Redmond D.L., Knox D.P. 2004. Protection studies in sheep using affinity-purified and recombinant cysteine proteinases of adult Haemonchus contortus. Vaccine, 22, 4252–4261. DOI: http://dx.doi.org/ 10.1016/j.vaccine.2004.04.028.
• Robinson Y., Shoichiro O. 2006. Dual roles of tropomysin as an Factin stabilizer and aregulator of muscle contraction in Caenorhabditis elegans body wall muscle. Cell Motility and the Cytoskeleton, 63, 659–672. DOI: 10.1002/cm.20152.
• Skuce P.J., Redmond D.L., Liddell S., Stewart E.M., Newlands G.F., Smith W.D., Knox D.P. 1999. Molecular cloning and characterization of gut-derived cysteine proteinases associated with a host protective extract from Haemonchus contortus. Parasitology, 119, 405–412.
• Smith T.S., Munn E.A. 1990. Strategies for vaccination against gastro-intestinal nematodes. Revue scientifique et technique, 9, 577–595.
• Smith W.D., Smith S.K., Murray J.M. 1994. Protection studies with integral membrane fractions of Haemonchus contortus. Parasite Immunology, 16, 231–241. DOI: http://dx.doi.org/ 10.1111/j.1365-3024.1994.tb00345.x.
• Song H., Yan R., Xu L., Song X., Shah M.A., Zhu H., Li X. 2010. Efficacy of DNA vaccines carrying Eimeria acervulina lactate dehydrogenase antigen gene against coccidiosis. Experiment Parasitology, 126, 224–231. DOI: http://dx.doi.org/ 10.1016/j.exppara.2010.05.015.
• Song X., Xu L., Yan R., Huang X., Shah M.A., Li X. 2009. The optimal immunization procedure of DNA vaccine pcDNATA4-IL-2 of Eimeria tenella and its cross-immunity to Eimeria necatrix and Eimeria acervulina. Veterinary Parasitology, 159, 30–36. DOI: http://dx.doi.org/ 10.1016/j.vetpar.2008.10.015.
• Stear M.J., Bishop S.C., Doligalska M., Duncan J.L., Holmes P.H., Irvine J., McCririe L., McKellar Q.A., Sinski E., Murray M. 1995. Regulation of egg production, worm burden, worm length and worm fecundity by host responses in sheep infected with Ostertagia circumcincta. Parasite Immunology, 17, 643–652. DOI: http://dx.doi.org/ 10.1111/j.1365-3024.1995.tb01010.x.
• Sun W., Song X., Yan R., Xu L., Li X. 2011. Vaccination of goats with a glutathione peroxidase DNA vaccine induced partial protection against Haemonchus contortus infection. Veterinary Parasitology, 182, 239–247. DOI: http://dx.doi.org/ 10.1016/j.vetpar.2011.05.024.
• Sun Y., Yan R., Muleke C.I., Zhao G., Xu L., Li X. 2007. Vaccination of goats with recombinant galectin antigen induces partial protection against Haemonchus contortus infection. Parasite Immunology, 29, 319–326. DOI: http://dx.doi.org/ 10.1111/j.1365-3024.2007.00949.x.
• Tang D.C., Devit M., Johnston S.A. 1992. Genetic immunization is a simple method for eliciting an immune response. Nature, 356, 152–154. DOI: http://dx.doi.org/ 10.1038/356152a0.
• Winter M.D., Wright C., Lee D.L. 1997. The mast cell and eosinophil response of young lambs to a primary infection with Nematodirus battus. Parasitology, 114, 189–193. DOI: http://dx.doi.org/ 10.1017/S0031182096008311.
• Wolstenholme A.J., Fairweather I., Prichard R., von Samson-Himmelstjerna G., Sangster N.C. 2004. Drug resistance in veterinary helminths. Trends in Parasitology, 20, 469–476. DOI: http://dx.doi.org/ 10.1016/j.pt.2004.07.010.
• Yacob H.T., Dorchies P., Jacquiet P., Bleuart C., Prevot F., Grisez C., Bergeaud J.P., Hoste H. 2004. Concurrent parasitic infections of sheep: depression of Trichostrongylus colubriformis populations by a subsequent infection with Oestrus ovis. Veterinary Parasitology, 121, 297–306. DOI: http://dx.doi.org/ 10.1016/j.vetpar.2004.01.013.
• Yan F., Xu L., Liu L., Yan R., Song X., Li X. 2010 Immunoproteomic analysis of whole proteins from male and female adult Haemonchus contortus. The Veterinary Journal, 185, 174–179. DOI: http://dx.doi.org/ 10.1016/j.tvjl.2009.05.021.
• Yan R., Yan F., Wang J., Xu L., Song X., Li X. 2012. Gene cloning, expression and DNA vaccine construction of Haemonchus contortus Actin. Journal of Nanjing Agricultural University, 35, 89–96.
• Yan R., Sun W., Song X., Xu L., Li X. 2013. Vaccination of goats with DNA vaccine encoding Dim-1 induced partial protection against Haemonchus contortus: A preliminary experimental study. Research in Veterinary Science, 95, 189–199. DOI: http://dx.doi.org/ 10.1016/j.rvsc.2013.02.020.
• Zhao G., Yan R., Muleke C.I., Sun Y., Xu L., Li X. 2012. Vaccination of goats with DNA vaccines encoding H11 and IL-2 induces partial protection against Haemonchus contortus infection. The Veterinary Journal, 191, 94–100. DOI: http://dx.doi.org/ 10.1016/j.tvjl.2010.12.023.
• Zhou H., Min J., Zhao Q., Gu Q., Cong H., Li Y., He S. 2012. Protective immune response against Toxoplasma gondii elicited by a recombinant DNA vaccine with a novel genetic adjuvant. Vaccine, 30, 1800–1806. DOI: http://dx.doi.org/ 10.1016/j.vaccine.2012.01.004.