In vitro cultivation and immunostaining of Lawsonia intracellularis strains

• Autorzy: Szczotka-Bochniarz A. , Podgórska K. , Nowak A. , Pejsak Z.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to implement in vitro cultivation of L. intracellularis strains using ATCC 55783 and vaccine strains, and McCoy cells (ATCC CRL-1696). The infection was monitored by daily observations under phase contrast microscope. Indirect immunostaining using monoclonal antibody was also performed. Large number of S-shaped, moving bacteria were found in the cell medium in cultures infected with ATCC 55783 and vaccine strain. Immunostaining revealed a high number of multiple cell-associated or intracellular red stained bacteria in the infected cultures. This study describes for the first time in vitro cultivation of L. intracellularis in Poland, which creates further perspective for more advanced research on this bacterium.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 3
• Opis fizyczny: p.319-322,fig.,ref.

Twórcy

autor

Szczotka-Bochniarz A.

• Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Podgórska K.

• Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Nowak A.

• Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Pejsak Z.

• Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

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Identyfikatory

DOI

10.2478/bvip-2013-0055