NO-sGC signaling in rat cervical spinal cord after unilateral spinal cord injury

• Autorzy: Capkova L. , Davidova A. , Kuchankova A. , Pavel J. , Lukacova N.
• Języki publikacji: EN

Abstrakty

EN

The NO-sensitive guanylyl cyclase (sGC) as a major type of cyclic guanosine monophosphate (cGMP) - forming enzymes represents the best characterized receptor for the signal molecule nitric oxide (NO) produced by the NO synthases. Relatively high levels of the sGC are found in lung, brain and platelets, but little is known about the NO-cGMP signalling in the spinal cord. Our study was focused on NO-sGC signalization in bulbospinal respiratory pathway. We used a model of spinal cord hemisection performed at C2-C3 segmental level. After spinal cord injury the animals (adult rats) survived for eight days. The expression of neuronal nitric oxide synthase (nNOS) and β1 subunit of sGC was identified by immunohistochemical fluorescent method in ventral respiratory group (VRG) of medulla and in phrenic nucleus (PN) located at C4-C5 segmental level. In addition, the retrograde tracer Fluorogold (FG) was used to label neurons of VRG. These neurons were nNOS-immunoreactive. Two days after FG injection into the phrenic nucleus (PN), we revealed many FG fluorescent neurons mostly contralaterally to the site of injection. In control group, we noted nNOSfluorescent terminals of VRG neurons around beta 1 soluble guanylyl cyclase (ß1sGC) fluorescent motoneurons in PN. Spinal cord hemisection caused a significant decrease of the nNOS- and Synaptophysin (SYN) fluorescent terminals around alfa motoneurons in contralateral PN 8 days after hemisection. On the side of injury, nNOS/SYN fluorescent puncta were detected around phrenic motoneurons only sporadically. Phrenic alfa motoneurons responded to C2-C3 hemisection by a loss of ß1sGC positivity. These results together suggest that bulbospinal respiratory pathway is acting through NO-sGC. The experimental work was supported by VEGA Grant 2/0015/08.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: 1
• Opis fizyczny: p.155

Twórcy

autor

Capkova L.

• Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia

autor

Davidova A.

• Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia

autor

Kuchankova A.

• Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia

autor

Pavel J.

• Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia

autor

Lukacova N.

• Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia