Highly efficient Agrobacterium-based transformation sysyem for callus cells of the C3 halophyte Suaeda salsa

• Autorzy: Zhao S.-Z. , Ruan Y. , Sun H.-Z. , Wang B.-S.
• Języki publikacji: EN

Abstrakty

EN

Genetic manipulation technologies have been limited in the halophyte Suaeda salsa L. due to the lack of an efficient transformation system. Here, we examined factors affecting transformation and developed an efficient transformation system at the cell level using S. salsa hypocotyl as starting material. S. salsa hypocotyl explants from 10-day-old seedlings were precultured for 2 days on a hygromycin (hyg)-free callus induction medium (CIM) and then inoculated with Agrobacterium tumefaciens suspension at a concentration of 0.5 at OD600 for 5–10 min. After cocultivation with A. tumefaciens for 4 days in the dark, followed by selection on carbenicillin (carb) for 3 days, explants were placed on CIM containing 10 mg l⁻¹ hyg and 500 mg l⁻¹-carb with three to four consecutive subcultures for up to 45 days. β-Glucuronidase assays showed an average transformation frequency of 62.89%. Gene integration was confirmed by polymerase chain reaction analysis and Northern blot analysis. To our knowledge, this is the first study to show Agrobacterium-mediated transformation in the C3 halophyte S. salsa.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 5
• Opis fizyczny: p.729-736,fig.,ref.

Twórcy

autor

Zhao S.-Z.

• Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, No. 88, East Wenhua Road, 250014 Jinan, People's Republic of China

autor

Ruan Y.

• Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, No. 88, East Wenhua Road, 250014 Jinan, People's Republic of China

autor

Sun H.-Z.

• Department of Biological Science and Technology, Shandong Institute of Education, No. 36 Lishan Road, 250013 Jinan, People's Republic of China

autor

Wang B.-S.

• Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, No. 88, East Wenhua Road, 250014 Jinan, People's Republic of China

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